Scholarlink

<p>This research focuses on assessing the larvicidal effectiveness of chlorpyrifos, λ-cyhalothrin, and azadirachtin against <i>Aedes aegypti</i>, a major vector of dengue fever. Larvicidal efficacy was assessed by determining LC₅₀ values at 12, 24 and 48&#xa0;h, revealing chlorpyrifos (0.187, 0.010 and 0.002&#xa0;ppm) as the most potent, followed by λ-cyhalothrin (0.309, 0.068 and 0.031&#xa0;ppm) and azadirachtin (9.671, 6.855 and 3.358&#xa0;ppm). The study also examined the pesticides' ovicidal capabilities, finding complete egg mortality at minimal concentrations for each (0.5&#xa0;ppm—chlorpyrifos, 2.5&#xa0;ppm—λ-cyhalothrin, and 7&#xa0;ppm– azadirachtin). Additionally, the impact of these substances on oxidative stress response in the larvae was investigated, observing significant changes in catalase and superoxide dismutase enzyme activities, as well as in levels of the oxidative stress marker, malondialdehyde. Using the General Unified Threshold of Survival (GUTS) model, survival rates over time were analysed to assess the broad lethal impacts of the pesticides. Histopathological studies revealed considerable damage to the midgut epithelial columnar cells and the peritrophic membrane of treated <i>A. aegypti</i> larvae indicating significant toxicological alterations. In molecular docking study, the potential inhibitory roles of these chemicals on CAT (3RE8_A) and SOD (6s0d_A) were observed. Furthermore, the BOILED-Egg model was used to predict the potential of the chemicals for gastrointestinal absorption and penetration of the blood–brain barrier in humans. It helped to assess their environmental impact and effects on human health. Azadirachtin was found to be safer compared to the other two. Lastly, Generalized Read-Across (GenRA) analysis identified common toxicity pathways between the studied pesticides and their analogues. This comprehensive evaluation provides critical insights into the efficacy and mechanisms of action of these pesticides against <i>A. aegypti</i>, offering valuable information for the development of more effective and sustainable mosquito control strategies.</p> Graphical Abstract <p></p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Biopesticide (Azadirachtin) as a Sustainable Alternative to Conventional Pesticides (Chlorpyrifos and λ-Cyhalothrin) for Mosquito Control in the Dengue Vector, Aedes aegypti: Insights from Physiological, Histopathological and Molecular Docking Analyses

  • 期刊论文

  • 发表日期：

    2025年7月25日

查看全文
  • Souvik Bag,
  • Manabendu Barman,
  • Ayan Mondal,
  • Debraj Biswal,
  • Shubhajit Saha,
  • Soumendranath Chatterjee,
  • Sanjib Ray

摘要

This research focuses on assessing the larvicidal effectiveness of chlorpyrifos, λ-cyhalothrin, and azadirachtin against Aedes aegypti, a major vector of dengue fever. Larvicidal efficacy was assessed by determining LC₅₀ values at 12, 24 and 48 h, revealing chlorpyrifos (0.187, 0.010 and 0.002 ppm) as the most potent, followed by λ-cyhalothrin (0.309, 0.068 and 0.031 ppm) and azadirachtin (9.671, 6.855 and 3.358 ppm). The study also examined the pesticides' ovicidal capabilities, finding complete egg mortality at minimal concentrations for each (0.5 ppm—chlorpyrifos, 2.5 ppm—λ-cyhalothrin, and 7 ppm– azadirachtin). Additionally, the impact of these substances on oxidative stress response in the larvae was investigated, observing significant changes in catalase and superoxide dismutase enzyme activities, as well as in levels of the oxidative stress marker, malondialdehyde. Using the General Unified Threshold of Survival (GUTS) model, survival rates over time were analysed to assess the broad lethal impacts of the pesticides. Histopathological studies revealed considerable damage to the midgut epithelial columnar cells and the peritrophic membrane of treated A. aegypti larvae indicating significant toxicological alterations. In molecular docking study, the potential inhibitory roles of these chemicals on CAT (3RE8_A) and SOD (6s0d_A) were observed. Furthermore, the BOILED-Egg model was used to predict the potential of the chemicals for gastrointestinal absorption and penetration of the blood–brain barrier in humans. It helped to assess their environmental impact and effects on human health. Azadirachtin was found to be safer compared to the other two. Lastly, Generalized Read-Across (GenRA) analysis identified common toxicity pathways between the studied pesticides and their analogues. This comprehensive evaluation provides critical insights into the efficacy and mechanisms of action of these pesticides against A. aegypti, offering valuable information for the development of more effective and sustainable mosquito control strategies.

Graphical Abstract