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<p>Fiber-based colorimetric biosensors combine the high surface area, porosity, and transport properties of fibrous materials with the simplicity and visual accessibility of colorimetric detection. Of these platforms, nanofiber-based systems have attracted increasing attention due to their tunable morphology, which enhances analyte diffusion and accelerates chromogenic responses. Nevertheless, despite the rapid growth of colorimetric biosensing, nanofiber-mediated colorimetric systems remain comparatively underexplored, and critical questions related to reproducibility, robustness, and scalability remain unresolved. This review examines the current state of nanofiber-based colorimetric biosensors from the perspective of materials and design, considering how fabrication strategies, surface functionalization, and emerging readout approaches shape the analytical performance of these biosensors in various applications. By critically assessing current limitations alongside recent advances, the review aims to clarify the opportunities and constraints of nanofiber colorimetric platforms and to outline realistic directions for their future development.</p>

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Engineering Fiber-Based Colorimetric Biosensors Toward Integrated Analytical Platforms

  • 期刊论文

  • 发表日期：

    2026年7月11日

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  • Hichem Moulahoum,
  • Emine Guler Celik,
  • Tuncay Goksel,
  • Figen Zihnioglu,
  • Suna Timur,
  • Faezeh Ghorbanizamani

摘要

Fiber-based colorimetric biosensors combine the high surface area, porosity, and transport properties of fibrous materials with the simplicity and visual accessibility of colorimetric detection. Of these platforms, nanofiber-based systems have attracted increasing attention due to their tunable morphology, which enhances analyte diffusion and accelerates chromogenic responses. Nevertheless, despite the rapid growth of colorimetric biosensing, nanofiber-mediated colorimetric systems remain comparatively underexplored, and critical questions related to reproducibility, robustness, and scalability remain unresolved. This review examines the current state of nanofiber-based colorimetric biosensors from the perspective of materials and design, considering how fabrication strategies, surface functionalization, and emerging readout approaches shape the analytical performance of these biosensors in various applications. By critically assessing current limitations alongside recent advances, the review aims to clarify the opportunities and constraints of nanofiber colorimetric platforms and to outline realistic directions for their future development.