<p>The practicality of the non-enzymatic glucose sensing depends on the development of cost-effective, binder-free, and highly selective electrode catalysts. In this work, we successfully synthesized a porous Au₉₅Ru₅ solid-solution alloy electrode on a gold screen-printed electrode (Au-SPE) via one-step electrodeposition and dynamic hydrogen bubble templating (DHBT). This porous Au₉₅Ru₅ catalyzing electrode possesses high active surface areas and optimized electron transfer characteristics, thereby significantly enhancing their catalytic activity for glucose oxidation. As a result, the porous Au₉₅Ru₅ electrodes demonstrated superior glucose sensing capabilities, with a low detection limit of 2.1 µM and an impressive sensitivity of 5.46&#xa0;mA/(mM· cm²). They also show excellent selectivity against common interferents, as well as good stability and reproducibility over extended periods. This exceptional performance is attributed to the introduction of additional active sites through the incorporation of ruthenium (Ru) into gold (Au), which promotes the glucose oxidation reaction. The synergistic effect between Ru and Au reduces the reaction overpotential and enhances the current response. Concurrently, the high conductivity of Au is retained, ensuring rapid electron transfer, minimizing electrode polarization, and improving both the response speed and sensitivity of the sensor. Furthermore, Ru suppresses the accumulation of intermediate products and prevents catalyst surface poisoning, thereby maintaining long-term stability.</p>