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<p>Natural laccase is a copper-dependent polyphenol oxidase with broad substrate specificity and high catalytic efficiency toward phenolic compounds, making it an attractive biocatalyst for biosensing applications. In recent years, copper-based nanozymes have emerged as promising laccase mimics by simulating the active-site structure and catalytic functions of the natural enzyme. This review summarizes recent advances in the rational design and synthesis of copper-based laccase-like nanozymes, focusing on the critical roles of coordination atoms and their local microenvironments in modulating catalytic activity. Moreover, we discuss strategies for enhancing the performance of these nanozymes and highlight their applications in analytical detection, including the sensing of anions, pesticides, antibiotics, enzymes, phenolic compounds, and other environmental pollutants. Finally, we outline current challenges and future directions for the development of high-efficiency copper-based nanozymes, emphasizing their potential as robust and versatile tools in environmentally friendly biosensing platforms.</p>

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Design and Structural Regulation of Copper-based Laccase Nanozymes for Sensing Applications

  • 期刊论文

  • 发表日期：

    2026年2月26日

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  • Longhua Ding,
  • Mingyue Du,
  • Qingzhao Ping,
  • Yawen Wang,
  • Aizhu Wang,
  • Renliang Huang,
  • Xin Yu

摘要

Natural laccase is a copper-dependent polyphenol oxidase with broad substrate specificity and high catalytic efficiency toward phenolic compounds, making it an attractive biocatalyst for biosensing applications. In recent years, copper-based nanozymes have emerged as promising laccase mimics by simulating the active-site structure and catalytic functions of the natural enzyme. This review summarizes recent advances in the rational design and synthesis of copper-based laccase-like nanozymes, focusing on the critical roles of coordination atoms and their local microenvironments in modulating catalytic activity. Moreover, we discuss strategies for enhancing the performance of these nanozymes and highlight their applications in analytical detection, including the sensing of anions, pesticides, antibiotics, enzymes, phenolic compounds, and other environmental pollutants. Finally, we outline current challenges and future directions for the development of high-efficiency copper-based nanozymes, emphasizing their potential as robust and versatile tools in environmentally friendly biosensing platforms.