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<p>Given the growing concerns about foodborne diseases, there is an urgent need for rapid, sensitive, and specific pathogen detection in food. In this study, a magnetic phage probe-based Quartz Crystal Microbalance (QCM) biosensor was developed for the detection of Salmonella (<i>S.T</i>) as a model pathogen. The sensor utilizes Fe<sub>3</sub>O<sub>4</sub> nanoparticles modified with <i>S.T</i>-specific phages to selectively enrich and isolate live <i>S.T</i> from complex food matrices. To further enhance sensitivity, a novel enzyme amplification strategy using poly(IgG-HRP)<sub>n</sub> tags was employed. When combined with magnetic phage probes, these tags facilitate the formation of sandwich complexes on the QCM surface, allowing the 3,3’-Diaminobenzidine(DAB)-H<sub>2</sub>O<sub>2</sub> system to generate a large number of precipitates that induce amplified frequency shifts (∆<i>f</i>) in the QCM. These shifts are directly proportional to the concentration of <i>S.T</i>. The biosensor demonstrated high specificity and catalytic efficiency, with a detection range spanning from 10 to 1 × 10<sup>8</sup> CFU/mL and a detection limit of 3 CFU/mL. The results correlated well with standard plate counting methods, with recovery rates ranging from 92.3% to 110% in milk, egg and pork samples. This phage-based QCM sensor, coupled with poly(IgG-HRP)<sub>n</sub> tags, provides a sensitive, portable, and selective solution for the on-site detection of food pathogen.</p>

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Magnetic Phage-Based Quartz Crystal Microbalance Biosensor with Poly(IgG-HRP)n Tag: A Sensitive and Signal-amplified Detection Strategy for Salmonella in Foods

  • 期刊论文

  • 发表日期：

    2025年11月15日

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  • Tingting He,
  • Tingfeng Ma,
  • Xixue Chen,
  • Iren Kuznetsova,
  • Tianhua Li,
  • Peng Li,
  • Zhenghua Qian,
  • Vladimir Kolesov,
  • Shengfeng Huang,
  • Ning Gan

摘要

Given the growing concerns about foodborne diseases, there is an urgent need for rapid, sensitive, and specific pathogen detection in food. In this study, a magnetic phage probe-based Quartz Crystal Microbalance (QCM) biosensor was developed for the detection of Salmonella (S.T) as a model pathogen. The sensor utilizes Fe3O4 nanoparticles modified with S.T-specific phages to selectively enrich and isolate live S.T from complex food matrices. To further enhance sensitivity, a novel enzyme amplification strategy using poly(IgG-HRP)n tags was employed. When combined with magnetic phage probes, these tags facilitate the formation of sandwich complexes on the QCM surface, allowing the 3,3’-Diaminobenzidine(DAB)-H2O2 system to generate a large number of precipitates that induce amplified frequency shifts (∆f) in the QCM. These shifts are directly proportional to the concentration of S.T. The biosensor demonstrated high specificity and catalytic efficiency, with a detection range spanning from 10 to 1 × 108 CFU/mL and a detection limit of 3 CFU/mL. The results correlated well with standard plate counting methods, with recovery rates ranging from 92.3% to 110% in milk, egg and pork samples. This phage-based QCM sensor, coupled with poly(IgG-HRP)n tags, provides a sensitive, portable, and selective solution for the on-site detection of food pathogen.