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<p>In view of the importance of quantification of beta-amyloid (Aβ), an “aptamer -target- peptide” sandwich electrochemical biosensor to assay Aβ42 was fabricated by using gold–palladium bimetallic nanoparticles covalent organic framework-carbon cloth (Au–Pd@COF<sub>TTF-Thi</sub>-CC) and prion protein (PrP<sup>C</sup>) labeled with horseradish peroxidase (HRP) nanocomposites. The concentration of Aβ42 showed a positive correlation with the peak current resulting from the oxidation of hydroquinone (HQ) by HRP. In contrast, the reference current signal from thionin (Thi), derived from covalent organic framework (COF), showed a negative correlation. The current response of the electrochemical biosensor was proportional to the Aβ concentrations ranging from 0.01 to 2.5&#xa0;nM with a detection limit (LOD) of 2.17 fM (<i>S</i>/<i>N</i> = 3). The sensitivity of the sandwich electrochemical biosensor could be primarily amplified based on the substrates of Au–Pd@COF<sub>TTF-Thi</sub>-CC. The AuNPs and PdNPs not only facilitated the bonding of the linker based on the Au–S bond but also improved the performance of COF<sub>TTF-Thi</sub>-CC. Secondary sensitivity amplification of the biosensor could be achieved by using PrP<sup>C</sup>-labeled HRP to catalyze hydroquinone in solution. Furthermore, the biosensor showed excellent selectivity, anti-interference performance, and stability. The Aβ concentrations in cerebrospinal fluid (CSF) were successfully evaluated using the constructed biosensor. This assay provides a potential tool for early-stage Alzheimer's disease diagnosis.</p>

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Construction of an “aptamer-target-peptide” Sandwich Electrochemical Biosensor for Ultrasensitive Assay of Amyloid-Beta Oligomers based on Bimetallic Covalent Organic Framework

  • 期刊论文

  • 发表日期：

    2025年7月29日

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  • Yubing Lv,
  • Yanli Zhou,
  • Chaoran Lv,
  • Mengyao Wang,
  • Nan Sheng,
  • Yuanyuan Lin,
  • Cancan Wang,
  • Maotian Xu,
  • Jing Zhang,
  • Mei Yan

摘要

In view of the importance of quantification of beta-amyloid (Aβ), an “aptamer -target- peptide” sandwich electrochemical biosensor to assay Aβ42 was fabricated by using gold–palladium bimetallic nanoparticles covalent organic framework-carbon cloth (Au–Pd@COFTTF-Thi-CC) and prion protein (PrPC) labeled with horseradish peroxidase (HRP) nanocomposites. The concentration of Aβ42 showed a positive correlation with the peak current resulting from the oxidation of hydroquinone (HQ) by HRP. In contrast, the reference current signal from thionin (Thi), derived from covalent organic framework (COF), showed a negative correlation. The current response of the electrochemical biosensor was proportional to the Aβ concentrations ranging from 0.01 to 2.5 nM with a detection limit (LOD) of 2.17 fM (S/N = 3). The sensitivity of the sandwich electrochemical biosensor could be primarily amplified based on the substrates of Au–Pd@COFTTF-Thi-CC. The AuNPs and PdNPs not only facilitated the bonding of the linker based on the Au–S bond but also improved the performance of COFTTF-Thi-CC. Secondary sensitivity amplification of the biosensor could be achieved by using PrPC-labeled HRP to catalyze hydroquinone in solution. Furthermore, the biosensor showed excellent selectivity, anti-interference performance, and stability. The Aβ concentrations in cerebrospinal fluid (CSF) were successfully evaluated using the constructed biosensor. This assay provides a potential tool for early-stage Alzheimer's disease diagnosis.