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Objectives <p>To evaluate the synergistic effect of Leukocyte and Platelet-Rich Fibrin(L-PRF) and Photobiomodulation (PBM) on the migration of MG-63 osteoblastic cells.</p> Settings and design <p>In- vitro comparative study</p> Methods and material <p>L-PRF was prepared from peripheral blood obtained from six healthy individuals using Choukroun’s protocol. Conditioned media (20%) obtained from L-PRF membranes was utilized in a Transwell migration assay. MG-63 cells were divided into three groups: control (2% FBS), L-PRF-treated, and L-PRF + PBM group was exposed to diode laser irradiation (810 ± 10&#xa0;nm, 0.1W power, 60&#xa0;s 6&#xa0;J/cm<sup>2</sup>) in a non-contact, continuous mode. After a 24-h incubation, cells that migrated to the lower membrane surface were fixed, stained with 0.5% crystal violet, and quantified using ImageJ software.</p> <p>Statistical analysis.</p> <p>Statistical analysis was performed using one-way ANOVA with Tukey’s post hoc test.</p> Results <p>L-PRF treatment alone resulted in a 92% increase in osteoblast migration when compared to controls. The combined application of L-PRF and PBM produced a 184% increase in migration compared to the control group, indicating a statistically significant synergistic effect.</p> Conclusions <p>The findings demonstrate complimentary use of L-PRF and PBM substantially increases osteoblast migration, suggesting their potential for combined use in clinical bone regeneration strategies.</p>

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Synergistic effects of leucocyte and platelet-rich fibrin and photobiomodulation on migration of MG-63 osteoblastic cell line: an in vitro study

  • 期刊论文

  • 发表日期：

    2026年5月2日

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  • Pushpa S Pudakalkatti,
  • Viswendra Chikkam,
  • Sachita S Naik,
  • Kasthuri B K,
  • Vidyashri N Jugale,
  • Shiphalika Sinha

摘要

Objectives

To evaluate the synergistic effect of Leukocyte and Platelet-Rich Fibrin(L-PRF) and Photobiomodulation (PBM) on the migration of MG-63 osteoblastic cells.

Settings and design

In- vitro comparative study

Methods and material

L-PRF was prepared from peripheral blood obtained from six healthy individuals using Choukroun’s protocol. Conditioned media (20%) obtained from L-PRF membranes was utilized in a Transwell migration assay. MG-63 cells were divided into three groups: control (2% FBS), L-PRF-treated, and L-PRF + PBM group was exposed to diode laser irradiation (810 ± 10 nm, 0.1W power, 60 s 6 J/cm2) in a non-contact, continuous mode. After a 24-h incubation, cells that migrated to the lower membrane surface were fixed, stained with 0.5% crystal violet, and quantified using ImageJ software.

Statistical analysis.

Statistical analysis was performed using one-way ANOVA with Tukey’s post hoc test.

Results

L-PRF treatment alone resulted in a 92% increase in osteoblast migration when compared to controls. The combined application of L-PRF and PBM produced a 184% increase in migration compared to the control group, indicating a statistically significant synergistic effect.

Conclusions

The findings demonstrate complimentary use of L-PRF and PBM substantially increases osteoblast migration, suggesting their potential for combined use in clinical bone regeneration strategies.