Purpose <p>Labial frenectomy is a surgical procedure, in which the frenum, along with its attachments to the periosteum and bone is removed. The purpose of the current study was to compare the 980-nm diode Laser and conventional scalpel technique in maxillary labial frenectomy.</p> Methods <p>Thirty-two healthy patients with abnormally positioned frenums were enrolled. The participants were matched for gender, age group, and frenum classification. They were equally allocated to either 980-nm diode Laser group or conventional scalpel technique group. Post-operative assessments were carried out at 2–4 days, 10 days, one month, and three months. Intra-operative indices were assessed including difficulty and duration of the procedure, and bleeding. In addition, post-operative indices were evaluated including pain perception, wound healing, edema, scar formation, and amount of attached gingival width gain.</p> Results <p>Results of bleeding index, and procedure duration showed significantly better results in laser’s group when compared with the scalpel group, p-values were 0.00, and 0.007, respectvely. Diode laser group showed significantly lower pain values at the first three timepoints (as p-value was 0.00 for all). At 9–10 days and one-month post-operative evaluation, healing scores did not reveal any significant differences (p-values were 0.083, and 0.878, respectively). Laser group showed significantly better results regarding scar formation after one month (<i>p</i> = 0.022), and three months (<i>p</i> = 0.005).</p> Conclusion <p>With the limitations of the current study, the 980-nm Diode laser demonstrated several clinical advantages over the scalpel technique. It reduced the intra-operative bleeding, procedure duration, and post-operative pain and edema, while also improving the scar formation. It is a safe and an effective alternative to the conventional surgical technique.</p>