Laser-photobiomodulation on reactive oxygen species production in titanium disc-containig pre-osteoblast cells culture

The therapeutic success of dental implants depends on the wound healing process. Bone matrix production by osteoblast cells around implant is pivotal in this process. Osteoblast cells reactive oxygen species (ROS) production and cell adhesion are the initial steps in osseointegration. The Objective the aim was to investigate the laser-photbiomodulation (L-PBM) effect (GaAlAs, λ = 830 nm) on ROS production and membrane lipid peroxidation (LPO) in osteoblast cells cultures seeded on titanium (Ti) disc-containing medium. Cell cultures.

Control group: without Ti disc containing-medium and Ti disc-containing groups, that were divided in four distinct subgroups: irradiated with an energy density of 2 J/cm², 4 J/cm², 6 J/cm², 8 J/cm², at irradiation intervals of 24, 48 and 72 h.

After irradiation, groups were evaluated on: 1- quantification of H₂O₂ and LPO; 2- photomicrographic record of cell adhesion/proliferation. Photomicrographs showed that L-PBM increased cell adhesion to Ti disc. There was an increase of H₂O₂ production in a dose-dependent manner and a decrease in LPO levels after 48 h.

Results indicate that L-PBM stimulates the production of H₂O₂, and this increase has no effect on the peroxidation of membrane lipids. L-PBM may be related to the enhancement of osteoblast adhesion to Ti disc surface.