<p>Almond anthracnose, caused by <i>Colletotrichum</i> species, is one of the main barriers to production because of the limited effectiveness of preventive and curative treatments. This study evaluated in vitro the susceptibility of <i>Colletotrichum godetiae</i>, <i>C. acutatum</i> and <i>C. gloeosporioides</i> to seven fungicides, including biopesticides, natural mineral substances and synthetic organic compounds. Mycelial growth inhibition and conidial germination were assessed, and EC₅₀ values were estimated using dose–response models. <i>Bacillus amyloliquefaciens</i> D747 showed a complete and consistent inhibition of mycelial growth and conidial germination in all isolates, indicating high potential as a biocontrol agent. Among synthetic organic fungicides, captan and dithianon showed stable, dose-dependent inhibition with low EC₅₀ values. Fluazinam showed high efficacy against <i>C. godetiae</i> and <i>C. gloeosporioides</i>, but variable efficacy against conidial germination, suggesting limitations in preventive control. Difenoconazole showed inconsistent results, especially against <i>C. acutatum</i>, suggesting a possible reduction in sensitivity during model fitting. Sulphur and aqueous extract from the germinated seeds of sweet <i>Lupinus albus</i>, on the other hand, showed limited antifungal activity. These results, although requiring validation in field studies, show that the effectiveness of fungicides depends on the active substance and the target species and underline the need for specific management strategies. Integration of biological agents and chemical fungicides with multiple sites of action, combined with rotation of modes of action, is necessary to improve disease control and reduce the risk of resistance.</p>