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<p>Cypress trees (<i>Cupressaceae</i>) are commonly planted in European urban landscapes but frequently affected by fungal diseases. In this study, we investigated fungal pathogens associated with <i>Thuja occidentalis</i> L. and <i>Chamaecyparis</i> species at different sites in Hungary. <i>Fusarium</i> isolates, tentatively identified through sequencing of the nuclear ribosomal internal transcribed spacer (ITS), were obtained from browning, necrotic tissues. For species-level resolution, monoconidial cultures were produced, and multilocus phylogenetic analysis was performed using partial translation elongation factor 1-α (<i>TEF</i>), RNA polymerase II largest (<i>RPB1</i>), and second-largest (<i>RPB2-1</i> and <i>RPB2-2</i>) subunits. Seven <i>Fusarium</i> species representing six species complexes were identified: <i>F. clavus</i>, which was the most frequently isolated species; <i>F. verticillioides</i>; <i>F. sambucinum</i>; <i>F. culmorum</i>; <i>F. inflexum</i>; <i>F. negundinis</i>; and <i>F. celtidicola</i>. To our knowledge, this study is the first to report these <i>Fusarium</i> taxa from <i>Thuja occidentalis</i> and <i>Chamaecyparis</i> sp. trees. Our findings expand the current knowledge on host range of several <i>Fusarium</i> taxa and contribute to a more comprehensive understanding of the diversity of <i>Fusarium</i> species associated with ornamental conifers.</p>

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Fusarium isolates representing six species complexes from symptomatic scale-leaved conifers (Cupressaceae)

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2026年7月21日

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  • Márk Z. Németh,
  • Diána Seress,
  • Gábor Bozsik,
  • József Fodor,
  • Orsolya Molnár

摘要

Cypress trees (Cupressaceae) are commonly planted in European urban landscapes but frequently affected by fungal diseases. In this study, we investigated fungal pathogens associated with Thuja occidentalis L. and Chamaecyparis species at different sites in Hungary. Fusarium isolates, tentatively identified through sequencing of the nuclear ribosomal internal transcribed spacer (ITS), were obtained from browning, necrotic tissues. For species-level resolution, monoconidial cultures were produced, and multilocus phylogenetic analysis was performed using partial translation elongation factor 1-α (TEF), RNA polymerase II largest (RPB1), and second-largest (RPB2-1 and RPB2-2) subunits. Seven Fusarium species representing six species complexes were identified: F. clavus, which was the most frequently isolated species; F. verticillioides; F. sambucinum; F. culmorum; F. inflexum; F. negundinis; and F. celtidicola. To our knowledge, this study is the first to report these Fusarium taxa from Thuja occidentalis and Chamaecyparis sp. trees. Our findings expand the current knowledge on host range of several Fusarium taxa and contribute to a more comprehensive understanding of the diversity of Fusarium species associated with ornamental conifers.