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<p>Barley stripe mosaic virus (BSMV), a significant pathogen of cereal crops, causes considerable yield losses worldwide. Despite the potential threat to cereal production, the biological and molecular characteristics of BSMV isolates in Ukraine remain largely unexplored. This study aimed to determine the incidence and distribution of BSMV in wheat during a five-year surveillance period (2020–2024) across major wheat-growing regions of Ukraine and to characterize the biological and molecular properties of obtained isolates. BSMV infection was confirmed by serological and molecular analyses in wheat fields of the Kyiv region in 2020 and 2024. Two isolates, designated BSMV-UA-2020 and BSMV-UA-2024, were identified and subjected to biological characterization. Both isolates exhibited high virulence, significantly affecting wheat development. Spike length was reduced by 31.6%, the number of seeds per spike decreased by 39.3%, thousand-grain weight declined by 2.2 times, and seed germination dropped by 90–94%. The restricted geographical distribution of BSMV in Ukraine may be explained by the high pathogenicity of local strains, which likely reduce seed viability and impairs seed-borne transmission. Phylogenetic analysis of the coat protein genes indicated 99.57% and 100% nucleotide identity with Polish isolates, suggesting a conserved regional lineage and possible transboundary transmission facilitated by agricultural trade or seed exchange. The partial coat protein gene sequences have been submitted to GenBank (accession numbers PV650257 and PV650258).</p>

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Biological characterization and phylogenetic analysis of Ukrainian barley stripe mosaic virus isolates

  • 期刊论文

  • 发表日期：

    2026年2月19日

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  • Tetiana Shevchenko,
  • Halyna Snihur,
  • Illia Pozhylov,
  • Anhelina Kyrychenko

摘要

Barley stripe mosaic virus (BSMV), a significant pathogen of cereal crops, causes considerable yield losses worldwide. Despite the potential threat to cereal production, the biological and molecular characteristics of BSMV isolates in Ukraine remain largely unexplored. This study aimed to determine the incidence and distribution of BSMV in wheat during a five-year surveillance period (2020–2024) across major wheat-growing regions of Ukraine and to characterize the biological and molecular properties of obtained isolates. BSMV infection was confirmed by serological and molecular analyses in wheat fields of the Kyiv region in 2020 and 2024. Two isolates, designated BSMV-UA-2020 and BSMV-UA-2024, were identified and subjected to biological characterization. Both isolates exhibited high virulence, significantly affecting wheat development. Spike length was reduced by 31.6%, the number of seeds per spike decreased by 39.3%, thousand-grain weight declined by 2.2 times, and seed germination dropped by 90–94%. The restricted geographical distribution of BSMV in Ukraine may be explained by the high pathogenicity of local strains, which likely reduce seed viability and impairs seed-borne transmission. Phylogenetic analysis of the coat protein genes indicated 99.57% and 100% nucleotide identity with Polish isolates, suggesting a conserved regional lineage and possible transboundary transmission facilitated by agricultural trade or seed exchange. The partial coat protein gene sequences have been submitted to GenBank (accession numbers PV650257 and PV650258).