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<p>The p3 protein is a viral suppressor of RNA silencing (VSR), encoded by the viral strand of RNA3 in the rice stripe virus (RSV). As a VSR, p3 facilitates viral infection, however, its broader functional impact on the host plant remains less clear. In this study, we employed transcriptome sequencing and RT-qPCR to analyze differentially expressed genes (DEGs) in transgenic rice expressing the RSV p3 gene. A total of 533 DEGs were identified between p3 transgenic and wild-type plants, including 214 upregulated and 319 downregulated genes. Among these, several NB-ARC (Os11G0492300, Os06G0268500, Os11G0227100) in this study were significantly upregulated. Expression of multiple kinase genes (Os10G0539600, Os03G0331700, Os05G0498900, Os02G0786900, Os03G0758250, Os04G0339800) were markedly altered, with the majority of them being down-regulated and brassinosteroid biosynthesis gene were uniquely expressed (Os03G0602300) or down-regulated (Os07G0162100) in Os_p3 plants. Given the complexity of plant–pathogen interactions and brassinosteroid signaling, this study highlights two potential pathways and establishes a foundation for future functional studies of candidate genes and specific pathway components. These findings offer novel insights into the sophisticated transcriptional reprogramming triggered by VSRs in host plants.</p>

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Transcriptome analysis of transgenic rice ectopically expressing the rice stripe virus p3 protein

  • 期刊论文

  • 发表日期：

    2026年2月27日

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  • Binhao Gao,
  • Long Ma,
  • Huiyuan Zhang,
  • Sinan Chen,
  • Qiao Wang,
  • Ling qing,
  • Gentu Wu

摘要

The p3 protein is a viral suppressor of RNA silencing (VSR), encoded by the viral strand of RNA3 in the rice stripe virus (RSV). As a VSR, p3 facilitates viral infection, however, its broader functional impact on the host plant remains less clear. In this study, we employed transcriptome sequencing and RT-qPCR to analyze differentially expressed genes (DEGs) in transgenic rice expressing the RSV p3 gene. A total of 533 DEGs were identified between p3 transgenic and wild-type plants, including 214 upregulated and 319 downregulated genes. Among these, several NB-ARC (Os11G0492300, Os06G0268500, Os11G0227100) in this study were significantly upregulated. Expression of multiple kinase genes (Os10G0539600, Os03G0331700, Os05G0498900, Os02G0786900, Os03G0758250, Os04G0339800) were markedly altered, with the majority of them being down-regulated and brassinosteroid biosynthesis gene were uniquely expressed (Os03G0602300) or down-regulated (Os07G0162100) in Os_p3 plants. Given the complexity of plant–pathogen interactions and brassinosteroid signaling, this study highlights two potential pathways and establishes a foundation for future functional studies of candidate genes and specific pathway components. These findings offer novel insights into the sophisticated transcriptional reprogramming triggered by VSRs in host plants.