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<p>Strawberries are vulnerable to diverse decay (rot) fungi. This research isolated two decay fungi <i>Botrytis deweyae</i> and <i>Aspergillus pseudotubingensis</i> from strawberry fruits and identified them based on sequencing data from the internal transcribed spacer (<i>ITS</i>) of ribosomal RNA (<i>ITS-rRNA</i>), RNA polymerase II second largest subunit (<i>RPB2</i>), and <i>ß-tubulin</i> (<i>BenA</i>) genes. The role of essential oils (EOs) in protracting the shelf-life of the fruits and attenuating disease severity (DS), was assessed by treating the fruits with combined thyme-rosemary essential oils (CTREOs). Next, CTREOs at 0%, 2.5%, 5%, and 10% v/v concentrations were encapsulated in electro-sprayed zein microcapsules (EZMs) and assessed for their potency against both fungi. The potency of CTREOs-loaded EZMs (at varying concentrations) to alleviate DS was compared with that of Signum<sup><b>®</b></sup> fungicide (at the concentrations of 25, 50, and 100&#xa0;mg L<sup>−1</sup>). The main compounds of each EO alone were identified by GC-MS analysis. CTREOs-loaded EZMs were characterized by scanning electron microscopy (SEM), transmission electron microscopy (TEM), Fourier transform infrared spectroscopy (FT-IR), thermo-gravimetric analysis (TGA), and X-ray diffraction (XRD). As per results, CTREOs (10% v/v)-loaded EZMs applied on strawberries noticeably declined DS, much more superb than three Signum<sup>®</sup> concentrations, and further enhanced the durability and storability of the inoculated fruits for 10&#xa0;days.</p>

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Molecular identification of decay fungi Botrytis deweyae and Aspergillus pseudotubingensis on strawberries, decay control, and fruit durability enhancement by combined thyme-rosemary microencapsulated essential oils

  • 期刊论文

  • 发表日期：

    2025年4月19日

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  • Samira Namazi,
  • Mahdi Pirnia,
  • Mohammad Amin Miri,
  • Mojtaba Keykhasaber,
  • Shirahmad Sarani,
  • Fakhtak Taliei

摘要

Strawberries are vulnerable to diverse decay (rot) fungi. This research isolated two decay fungi Botrytis deweyae and Aspergillus pseudotubingensis from strawberry fruits and identified them based on sequencing data from the internal transcribed spacer (ITS) of ribosomal RNA (ITS-rRNA), RNA polymerase II second largest subunit (RPB2), and ß-tubulin (BenA) genes. The role of essential oils (EOs) in protracting the shelf-life of the fruits and attenuating disease severity (DS), was assessed by treating the fruits with combined thyme-rosemary essential oils (CTREOs). Next, CTREOs at 0%, 2.5%, 5%, and 10% v/v concentrations were encapsulated in electro-sprayed zein microcapsules (EZMs) and assessed for their potency against both fungi. The potency of CTREOs-loaded EZMs (at varying concentrations) to alleviate DS was compared with that of Signum® fungicide (at the concentrations of 25, 50, and 100 mg L−1). The main compounds of each EO alone were identified by GC-MS analysis. CTREOs-loaded EZMs were characterized by scanning electron microscopy (SEM), transmission electron microscopy (TEM), Fourier transform infrared spectroscopy (FT-IR), thermo-gravimetric analysis (TGA), and X-ray diffraction (XRD). As per results, CTREOs (10% v/v)-loaded EZMs applied on strawberries noticeably declined DS, much more superb than three Signum® concentrations, and further enhanced the durability and storability of the inoculated fruits for 10 days.