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<p>The pinewood nematode, <i>Bursaphelenchus xylophilus</i>, is the causative agent of the pine wilt disease (PWD), which is an enormous threat to pine forests around the world. Once infected, pine trees may wither and die quickly. So, early detection of <i>B. xylophilus</i> is of great importance for monitoring and control of PWD. In this work, a portable microfluidic system is developed for <i>B. xylophilus</i> detection. The DNA is extracted directly from pine wood dusts through boiling lysis. Then, the recombinase polymerase amplification (RPA) method is applied to amplify the target DNA. The sample is finally tested using a gold immunochromatography strip. The entire processes including temperature control, fluidic driving, valving, metering, and mixing are automatically executed. The result can be obtained within 40&#xa0;min. The system provides a convenient tool for field detection, and allows early warning of PWD.</p>

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A portable microfluidic system for Bursaphelenchus xylophilus detection

  • 期刊论文

  • 发表日期：

    2025年2月11日

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  • Y. Z. Sun,
  • C. Wang,
  • D. Z. Wang,
  • Y. S. Bao,
  • L. Zhu,
  • H. M. Xia

摘要

The pinewood nematode, Bursaphelenchus xylophilus, is the causative agent of the pine wilt disease (PWD), which is an enormous threat to pine forests around the world. Once infected, pine trees may wither and die quickly. So, early detection of B. xylophilus is of great importance for monitoring and control of PWD. In this work, a portable microfluidic system is developed for B. xylophilus detection. The DNA is extracted directly from pine wood dusts through boiling lysis. Then, the recombinase polymerase amplification (RPA) method is applied to amplify the target DNA. The sample is finally tested using a gold immunochromatography strip. The entire processes including temperature control, fluidic driving, valving, metering, and mixing are automatically executed. The result can be obtained within 40 min. The system provides a convenient tool for field detection, and allows early warning of PWD.