<p>This study provides a quantitative bibliometric assessment of <i>Sepia officinalis</i> research indexed in the Web of Science Core Collection between 1977 and 2025. A total of 3535 publications were retrieved without restrictions and analyzed using VOSviewer. Annual output showed marked fluctuations: fewer than 25 publications annually until the late 1980s, followed by steady growth through the 1990s, and a sharp rise after 2000, peaking at 170 publications in 2020. Research articles accounted for 85.5% (<i>n</i> = 3022), followed by proceedings papers (<i>n</i> = 199) and reviews (<i>n</i> = 169). The dominant Web of Science categories were Marine &amp; Freshwater Biology (<i>n</i> = 729), Zoology (<i>n</i> = 557), and Fisheries (<i>n</i> = 441), underscoring the field’s core disciplinary scope. Leading journals included Journal of Experimental Biology (<i>n</i> = 91), Marine Biology (<i>n</i> = 61) and Aquaculture (<i>n</i> = 49). Country-level co-authorship mapping identified the USA (<i>n</i> = 508, TLS = 442), France (<i>n</i> = 456, TLS = 405) and Spain (<i>n</i> = 233, TLS = 329) as global collaboration hubs, with top institutions being the Marine Biological Laboratory (<i>n</i> = 98, TLS = 188) and University of Algarve (<i>n</i> = 82, TLS = 124). Author citation analysis revealed Hanlon, R.T. (<i>n</i> = 64, 2,842 citations, TLS = 2,995), Dickel, L. (<i>n</i> = 38, 1,168 citations, TLS = 2,877) and Messenger, J.B. (<i>n</i> = 14, 880 citations, TLS = 1,247) as intellectual leaders. Keyword co-occurrence highlighted major themes in behavioral ecology (camouflage), biomaterials (chitosan, hydroxyapatite), and environmental stress (ocean acidification). Overall, <i>S. officinalis</i> research demonstrates an interdisciplinary profile bridging marine biology, materials science and environmental sciences, with strong regional clusters but limited intercontinental integration.</p>