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<p><i>Saprolegnia</i>, a ubiquitous oomycete, is the predominant causative agent of Saprolegniosis, a dermatological infection in fish, and is accountable for substantial economic losses within the aquaculture industry. The necessity for a novel and efficient drug against the <i>Saprolegnia</i> species is of significant importance, particularly under the ban on malachite green oxalate in numerous countries and its inappropriate alternatives, such as formalin. The study encompassed two primary phases. Firstly, <i>Saprolegnia</i> species were isolated and characterized in water samples using culturing, LCB staining, PCR, gel electrophoresis, and DNA sequencing. Secondly, the antifungal activity and chemical constituents of <i>Nostoc commune</i> were assessed via well-diffusion, MIC/MFC assays, and GC-MS. Throughout the characterization methods, <i>Saprolegnia australis</i> was identified as the mold species present in the water samples. Additionally, the antifungal properties of the <i>Nostoc commune</i> extract were found to be satisfactory, as evidenced by a 30mm zone of inhibition and an MIC value of 2.5 mg/ml. In the present study, the saprolegniosis pathogen, <i>Saprolegnia australis</i>, was successfully identified and isolated from the ponds in the Mazandaran region where <i>Oncorhynchus mykiss</i> was present, thus marking a significant milestone as the first documented instance of its presence in Iran. Furthermore, the study presented a novel and promising therapeutic agent with the potential to effectively alleviate and mitigate the occurrence of fish skin infections, thus offering a new approach to combat this growing concern in aquaculture.</p>

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Antifungal Potential of Nostoc Commune Extract against Saprolegnia Australis

  • 期刊论文

  • 发表日期：

    2025年10月18日

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  • Arash Azari,
  • Pedram Zahedi Mohammadi,
  • Maryam Gholizadeh,
  • Issa Gholampour Azizi,
  • Ehsan Nazifi

摘要

Saprolegnia, a ubiquitous oomycete, is the predominant causative agent of Saprolegniosis, a dermatological infection in fish, and is accountable for substantial economic losses within the aquaculture industry. The necessity for a novel and efficient drug against the Saprolegnia species is of significant importance, particularly under the ban on malachite green oxalate in numerous countries and its inappropriate alternatives, such as formalin. The study encompassed two primary phases. Firstly, Saprolegnia species were isolated and characterized in water samples using culturing, LCB staining, PCR, gel electrophoresis, and DNA sequencing. Secondly, the antifungal activity and chemical constituents of Nostoc commune were assessed via well-diffusion, MIC/MFC assays, and GC-MS. Throughout the characterization methods, Saprolegnia australis was identified as the mold species present in the water samples. Additionally, the antifungal properties of the Nostoc commune extract were found to be satisfactory, as evidenced by a 30mm zone of inhibition and an MIC value of 2.5 mg/ml. In the present study, the saprolegniosis pathogen, Saprolegnia australis, was successfully identified and isolated from the ponds in the Mazandaran region where Oncorhynchus mykiss was present, thus marking a significant milestone as the first documented instance of its presence in Iran. Furthermore, the study presented a novel and promising therapeutic agent with the potential to effectively alleviate and mitigate the occurrence of fish skin infections, thus offering a new approach to combat this growing concern in aquaculture.