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<p>Acclimatizing in vitro seedlings to the natural environment is crucial. High environmental stress kills many seedlings at this stage. This study aims to determine the effect of vitamin B1 (thiamine) administration at the acclimatization stage of the tissue culture method on the quality of <i>Kappaphycus alvarezii</i> cultivars. The research consisted of five treatments: T1 (1&#xa0;ml/L thiamine), T2 (2&#xa0;ml/L thiamine), T3 (3&#xa0;ml/L thiamine), T4 (4&#xa0;ml/L thiamine), and T5 (without thiamine), each with three replications. The parameters observed included morphology (color and texture), branch addition, seaweed growth, and water quality. The results showed that thiamine administration significantly affected the development and quality of <i>K. alvarezii</i>. A dose of thiamine of 3&#xa0;ml/L (T3) produced the best growth with an average weight increase of 1.8&#xa0;g, adding 21 branches. In addition, healthy physiological conditions were indicated by a dark brown color and a dense, chewy texture. Treatment T3 was followed by T2, T1, T4, and T5, which showed more limited growth enhancement and lower yields in all observed parameters. This study recommends using thiamine 3&#xa0;ml/L as an essential nutrient supplement in the acclimatization process of tissue-cultured <i>K. alvarezii</i>.</p>

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Optimizing Kappaphycus alvarezii Tissue Culture Acclimatization Through Vitamin B1 Enrichment

  • 期刊论文

  • 发表日期：

    2025年8月20日

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  • Rashaki Yumeina Masfat,
  • Lestari Lakhsmi Widowati,
  • Seto Windarto

摘要

Acclimatizing in vitro seedlings to the natural environment is crucial. High environmental stress kills many seedlings at this stage. This study aims to determine the effect of vitamin B1 (thiamine) administration at the acclimatization stage of the tissue culture method on the quality of Kappaphycus alvarezii cultivars. The research consisted of five treatments: T1 (1 ml/L thiamine), T2 (2 ml/L thiamine), T3 (3 ml/L thiamine), T4 (4 ml/L thiamine), and T5 (without thiamine), each with three replications. The parameters observed included morphology (color and texture), branch addition, seaweed growth, and water quality. The results showed that thiamine administration significantly affected the development and quality of K. alvarezii. A dose of thiamine of 3 ml/L (T3) produced the best growth with an average weight increase of 1.8 g, adding 21 branches. In addition, healthy physiological conditions were indicated by a dark brown color and a dense, chewy texture. Treatment T3 was followed by T2, T1, T4, and T5, which showed more limited growth enhancement and lower yields in all observed parameters. This study recommends using thiamine 3 ml/L as an essential nutrient supplement in the acclimatization process of tissue-cultured K. alvarezii.