Scholarlink

<p>The olive oil industry generates large quantities of residues, including olive mill wastewater (OMWW), olive pomace, and pruning biomass, whose management represents a major environmental challenge in Mediterranean regions. The development of integrated strategies for the valorization of these phenolic-rich by-products is therefore essential to improve resource efficiency and reduce environmental impacts. In this study, an integrated solid-state fermentation (SSF) approach was developed to convert olive mill by-products into spore-rich fungal biomass. Indigenous filamentous fungi were isolated from olive-derived substrates and screened for growth, sporulation, and phenolic tolerance. <i>Trichoderma virens</i> was identified as the most suitable strain, combining rapid colonization, high conidial productivity, and strong phenolic-removal performance. During aerobic treatment of OMWW, <i>T. virens</i> achieved 77.2% color removal, 71.2% reduction in total phenolic content, 79% COD removal, and 78% BOD₅ removal after 15 days at 10⁷ spores mL⁻¹. At laboratory scale, SSF using olive-based substrates produced up to 4 × 10⁸ spores g⁻¹ dry substrate within 5 days. Scale-up in a 40&#xa0;L Zymotis-2 bioreactor, combined with controlled aeration and hydric stress, increased sporulation to 1.2 × 10⁹ spores g⁻¹ dry substrate, yielding a dry spore-rich biomass suitable for further formulation. The integration of treated OMWW as a moistening agent with solid residues enabled simultaneous valorization of liquid and solid fractions within a single bioprocess. This approach provides a scalable pathway for converting olive mill by-products into candidate microbial bioinputs and supports circular bioresource management in Mediterranean agroecosystems.</p> Graphical abstract <p> </p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Integrated valorization of olive mill by-products through solid-state fermentation for pre-industrial production of Trichoderma virens bioinputs

  • 期刊论文

  • 发表日期：

    2026年6月29日

查看全文
  • Hanen Zaier,
  • Walid Chmingui,
  • Amira Gannar,
  • Ajmi Larbi

摘要

The olive oil industry generates large quantities of residues, including olive mill wastewater (OMWW), olive pomace, and pruning biomass, whose management represents a major environmental challenge in Mediterranean regions. The development of integrated strategies for the valorization of these phenolic-rich by-products is therefore essential to improve resource efficiency and reduce environmental impacts. In this study, an integrated solid-state fermentation (SSF) approach was developed to convert olive mill by-products into spore-rich fungal biomass. Indigenous filamentous fungi were isolated from olive-derived substrates and screened for growth, sporulation, and phenolic tolerance. Trichoderma virens was identified as the most suitable strain, combining rapid colonization, high conidial productivity, and strong phenolic-removal performance. During aerobic treatment of OMWW, T. virens achieved 77.2% color removal, 71.2% reduction in total phenolic content, 79% COD removal, and 78% BOD₅ removal after 15 days at 10⁷ spores mL⁻¹. At laboratory scale, SSF using olive-based substrates produced up to 4 × 10⁸ spores g⁻¹ dry substrate within 5 days. Scale-up in a 40 L Zymotis-2 bioreactor, combined with controlled aeration and hydric stress, increased sporulation to 1.2 × 10⁹ spores g⁻¹ dry substrate, yielding a dry spore-rich biomass suitable for further formulation. The integration of treated OMWW as a moistening agent with solid residues enabled simultaneous valorization of liquid and solid fractions within a single bioprocess. This approach provides a scalable pathway for converting olive mill by-products into candidate microbial bioinputs and supports circular bioresource management in Mediterranean agroecosystems.

Graphical abstract