<p>This study investigated the biochemical composition of <i>Pistacia lentiscus</i> seed oil and evaluated its therapeutic potential in accelerating cutaneous wound healing. The fixed oil was extracted from the seeds using the Soxhlet method and biochemically characterized by gas chromatography, which revealed a predominance of oleic and linoleic acids. Its wound-healing activity was then assessed in an experimental rat model. The results showed that the ointment formulated with <i>P. lentiscus</i> seed oil promoted wound healing more effectively than the reference treatment, Mebo. Biochemical analyses demonstrated a significant reduction in malondialdehyde levels, along with enhanced antioxidant enzymatic defenses, particularly catalase and superoxide dismutase activities, in the group treated with <i>P. lentiscus</i> seed oil compared with the control and reference groups. Histological examination confirmed these findings by revealing improved tissue repair in the treated animals. Overall, these results suggest that <i>P. lentiscus</i> seed oil is a promising natural product for enhancing the wound-healing process, likely through its antioxidant properties and beneficial fatty acid composition. Further investigations are needed to clarify the underlying molecular mechanisms and to validate its therapeutic efficacy through clinical studies.</p> Graphical abstract <p></p>