<p>This study assessed the impact of irrigation using raw and treated wastewater from the Faculty of Sciences Ain Chock wastewater treatment plant on the growth, nutritional quality, and physiological responses of <i>Raphanus sativus L.</i> Physicochemical characterization revealed high concentrations of NH₄⁺ (24.07 ± 1.12&#xa0;mg L⁻¹) and SO₄²⁻ (219 ± 8.3&#xa0;mg L⁻¹) in raw wastewater, which decreased significantly after treatment (BOD₅ decreased from 50 ± 2.1 to 24 ± 1.7&#xa0;mg L⁻¹). Seeds irrigated with raw wastewater exhibited markedly lower α-amylase activity (0.0025 ± 0.0006 IU· mg⁻¹ protein) compared to controls (0.0208 ± 0.0013 IU·mg⁻¹ protein). Treated wastewater caused a milder reduction by 27% (0.0150 ± 0.0012 IU·mg protein⁻¹). Succinate dehydrogenase (SDH) activity was higher in leaves than fruits under control conditions (0.998 ± 0.19 vs. 0.505 ± 0.10 IU· mg⁻¹ protein). Irrigation with raw wastewater decreased leaf SDH to 0.394 ± 0.09 IU·mg⁻¹ protein (− 72%), while treated wastewater maintained intermediate activity (0.693 ± 0.11 IU·mg protein⁻¹). In fruits, SDH activity declined to 0.238 ± 0.09 IU·mg⁻¹ protein under raw wastewater (− 44%) and to 0.340 ± 0.08 IU·mg⁻¹ protein under treated wastewater (− 30%). Nutritional analysis of edible tissues revealed elevated lead concentrations (2.8 ± 0.3&#xa0;mg kg⁻¹), slightly above WHO (2015) guidelines, while cadmium remained undetected. Overall, treated wastewater enhanced growth performance while minimizing stress responses compared to raw wastewater, supporting its potential as a sustainable alternative for irrigation in urban and ornamental agriculture.</p>

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Effects of raw and treated wastewater irrigation on the growth, physiological, and biochemical responses of Raphanus sativus L.

  • Lamiaa Belasri,
  • Ayoub Mardi,
  • Nihad Chakri,
  • Mehdi El Mellouki,
  • Fouad Amraoui,
  • Btissam El Amrani,
  • Samah Ait Benichou

摘要

This study assessed the impact of irrigation using raw and treated wastewater from the Faculty of Sciences Ain Chock wastewater treatment plant on the growth, nutritional quality, and physiological responses of Raphanus sativus L. Physicochemical characterization revealed high concentrations of NH₄⁺ (24.07 ± 1.12 mg L⁻¹) and SO₄²⁻ (219 ± 8.3 mg L⁻¹) in raw wastewater, which decreased significantly after treatment (BOD₅ decreased from 50 ± 2.1 to 24 ± 1.7 mg L⁻¹). Seeds irrigated with raw wastewater exhibited markedly lower α-amylase activity (0.0025 ± 0.0006 IU· mg⁻¹ protein) compared to controls (0.0208 ± 0.0013 IU·mg⁻¹ protein). Treated wastewater caused a milder reduction by 27% (0.0150 ± 0.0012 IU·mg protein⁻¹). Succinate dehydrogenase (SDH) activity was higher in leaves than fruits under control conditions (0.998 ± 0.19 vs. 0.505 ± 0.10 IU· mg⁻¹ protein). Irrigation with raw wastewater decreased leaf SDH to 0.394 ± 0.09 IU·mg⁻¹ protein (− 72%), while treated wastewater maintained intermediate activity (0.693 ± 0.11 IU·mg protein⁻¹). In fruits, SDH activity declined to 0.238 ± 0.09 IU·mg⁻¹ protein under raw wastewater (− 44%) and to 0.340 ± 0.08 IU·mg⁻¹ protein under treated wastewater (− 30%). Nutritional analysis of edible tissues revealed elevated lead concentrations (2.8 ± 0.3 mg kg⁻¹), slightly above WHO (2015) guidelines, while cadmium remained undetected. Overall, treated wastewater enhanced growth performance while minimizing stress responses compared to raw wastewater, supporting its potential as a sustainable alternative for irrigation in urban and ornamental agriculture.