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<p>Petroleum hydrocarbons are persistent environmental pollutants posing significant ecological risks due to their toxicity and accumulation in soils. This study aimed to isolate and characterize bacterial strains capable of biodegrading hydrocarbons from petroleum-contaminated soils collected in the Douleb, Semmama, and Tamsmida regions of Tunisia. Ten bacterial strains were initially isolated and identified through morphological and biochemical analyses. Among them, two strains demonstrated robust growth in a minimal medium with crude oil as the sole carbon source. Phylogenetic analysis based on 16&#xa0;S rRNA gene sequencing identified these strains as <i>Acinetobacter variabilis</i> and <i>Bacillus cereus</i>. Biochemical assays revealed that these strains exhibited lipase and protease enzymatic activities, important for hydrocarbon degradation, and displayed tolerance to elevated salt concentrations (up to 150&#xa0;g/L NaCl). Their hydrocarbon-degrading potential was evaluated in vitro by incubating them with crude oil, followed by GC-MS analysis of residual hydrocarbons. Results showed significant reduction or disappearance of aromatic, functionalized, and aliphatic hydrocarbons, with <i>Acinetobacter variabilis</i> displaying broader degradation capabilities, including aromatic compounds, and <i>Bacillus cereus</i> effectively degrading high-molecular-weight alkanes. Growth kinetics correlated with hydrocarbon depletion, confirming active biodegradation. These findings highlight the complementary roles of these bacterial strains and their potential application in sustainable bioremediation strategies for petroleum-contaminated soils, including saline environments.</p>

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Biodegradation of petroleum hydrocarbons by Acinetobacter variabilis and Bacillus cereus isolated from hydrocarbon-contaminated soils in Tunisia

  • 期刊论文

  • 发表日期：

    2025年12月16日

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  • Lamia Khannous,
  • Maroua Gdoura-Ben Amor,
  • Nour Elhouda Mathlouthi,
  • Fehria Mouna Messaoud,
  • Radhouane Gdoura,
  • Hafedh Rigane,
  • Sonda Guermazi-Toumi

摘要

Petroleum hydrocarbons are persistent environmental pollutants posing significant ecological risks due to their toxicity and accumulation in soils. This study aimed to isolate and characterize bacterial strains capable of biodegrading hydrocarbons from petroleum-contaminated soils collected in the Douleb, Semmama, and Tamsmida regions of Tunisia. Ten bacterial strains were initially isolated and identified through morphological and biochemical analyses. Among them, two strains demonstrated robust growth in a minimal medium with crude oil as the sole carbon source. Phylogenetic analysis based on 16 S rRNA gene sequencing identified these strains as Acinetobacter variabilis and Bacillus cereus. Biochemical assays revealed that these strains exhibited lipase and protease enzymatic activities, important for hydrocarbon degradation, and displayed tolerance to elevated salt concentrations (up to 150 g/L NaCl). Their hydrocarbon-degrading potential was evaluated in vitro by incubating them with crude oil, followed by GC-MS analysis of residual hydrocarbons. Results showed significant reduction or disappearance of aromatic, functionalized, and aliphatic hydrocarbons, with Acinetobacter variabilis displaying broader degradation capabilities, including aromatic compounds, and Bacillus cereus effectively degrading high-molecular-weight alkanes. Growth kinetics correlated with hydrocarbon depletion, confirming active biodegradation. These findings highlight the complementary roles of these bacterial strains and their potential application in sustainable bioremediation strategies for petroleum-contaminated soils, including saline environments.