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<p>Drought-resistant and high-carbohydrate agricultural biomass serves as an alternative feedstock for the generation of bioenergy and metabolites. Among these, <i>Opuntia ficus indica</i> (OFI) cladodes are considered valuable crops due to their ability to thrive in arid environments while contributing to the soil fertility enhancement through their high productivity in these regions. Therefore, this study aimed to investigate the potential of OFI cladodes as a carbon source for biohydrogen (H<sub>2</sub>) and volatile fatty acids (VFA) production via mesophilic dark fermentation (DF). Dilute acid pretreatment (DAP) of cladodes was conducted. The concentration of sulfuric acid (3%, v/v) provided an optimum yield of 27&#xa0;g/L reducing sugars (RS). Initial experiments performed in 125&#xa0;mL glass bottles with a pH range of 5.0 to 7.0 showed that an initial pH of 6.0 was the most effective for DF, achieving the highest H₂ yield (1.54&#xa0;mol&#xa0;H₂/mol RS) and a H₂ production rate of 44.53&#xa0;mL/L.h. The optimized batch condition was then applied in a 2&#xa0;L bioreactor with pH regulation for fermentative H<sub>2</sub> production and VFA recovery at different cladode hydrolysate concentrations (7–35&#xa0;g RS/L). Increasing the RS concentration to 25&#xa0;g/L led to the highest H<sub>2</sub> productivity of 485&#xa0;mL/L.h, with VFA concentration of 12.1&#xa0;g/L. The pattern of VFA revealed that butyrate and acetate were the dominant byproducts during the DF process, with the formation of butyrate as the dominant pathway. Consequently, prickly pear cladodes represent an effective substrate for advancing the circular economy and contributing to sustainable development goals by producing eco-friendly products, tackling both the climate change and water scarcity challenges.</p> Graphical abstract <p></p>

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Biohydrogen and volatile fatty acids production from prickly pear cladodes (Opuntia ficus indica) as renewable feedstock

  • 期刊论文

  • 发表日期：

    2025年2月28日

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  • Rafika Saidi,
  • Manel Ziadi,
  • Souhir Bouazizi,
  • Hassib Bouallagui,
  • Moktar Hamdi

摘要

Drought-resistant and high-carbohydrate agricultural biomass serves as an alternative feedstock for the generation of bioenergy and metabolites. Among these, Opuntia ficus indica (OFI) cladodes are considered valuable crops due to their ability to thrive in arid environments while contributing to the soil fertility enhancement through their high productivity in these regions. Therefore, this study aimed to investigate the potential of OFI cladodes as a carbon source for biohydrogen (H2) and volatile fatty acids (VFA) production via mesophilic dark fermentation (DF). Dilute acid pretreatment (DAP) of cladodes was conducted. The concentration of sulfuric acid (3%, v/v) provided an optimum yield of 27 g/L reducing sugars (RS). Initial experiments performed in 125 mL glass bottles with a pH range of 5.0 to 7.0 showed that an initial pH of 6.0 was the most effective for DF, achieving the highest H₂ yield (1.54 mol H₂/mol RS) and a H₂ production rate of 44.53 mL/L.h. The optimized batch condition was then applied in a 2 L bioreactor with pH regulation for fermentative H2 production and VFA recovery at different cladode hydrolysate concentrations (7–35 g RS/L). Increasing the RS concentration to 25 g/L led to the highest H2 productivity of 485 mL/L.h, with VFA concentration of 12.1 g/L. The pattern of VFA revealed that butyrate and acetate were the dominant byproducts during the DF process, with the formation of butyrate as the dominant pathway. Consequently, prickly pear cladodes represent an effective substrate for advancing the circular economy and contributing to sustainable development goals by producing eco-friendly products, tackling both the climate change and water scarcity challenges.

Graphical abstract