Scholarlink

<p>Emerging contaminants (ECs), including endocrine-disrupting chemicals, dyes, pharmaceuticals, and pesticides, are increasingly released into the environment due to untreated industrial discharges, agricultural runoff, and the inefficiency of conventional treatment methods. Even at trace concentrations, their persistence necessitates effective remedial measures. Conventional physical and chemical methods are often inefficient, costly, generate secondary pollutants. To overcome these challenges, enzyme-based technologies have gained attention as green alternatives owing to their high catalytic efficiency, substrate specificity, and ability to operate under mild conditions without generating secondary pollution. Ligninolytic enzymes such as laccase (Lac), lignin peroxidase (LiP), manganese peroxidase (MnP), versatile peroxidase (VP), and horseradish peroxidase (HRP), primarily derived from white-rot fungi, are promising biocatalyst for EC degradation due to their broad substrate specificity, high catalytic efficiency, and stability. However, poor operational stability, denaturation susceptibility, high production cost (USD 200–1000 per kg), and poor reusability (1–3 cycles) constrain the application of free enzymes. Consequently, enzyme immobilization has arisen as an effective strategy to overcome these problems. Nanoengineered supports for enzyme immobilization offer advantages such as high surface area, easy recovery, improved catalytic stability, and enzyme loading, reduced enzyme leaching, and easy magnetic separation. Nanoimmobilized ligninolytic enzymes exhibit 2–fivefold higher stability, retain 70–90% activity after 3–10 cycles, and achieve a 40–70% improvement in process economics. This review discusses recent advances in nanoengineered ligninolytic enzyme systems for EC remediation, emphasizing catalytic degradation mechanisms, immobilization strategies, enzyme-nanomaterial interactions, and factors influencing catalytic performance. It also highlights large-scale implementation of immobilized enzyme strategies for efficient EC removal and outlines future research directions.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Nanoengineered ligninolytic enzymes: a promising tool for efficient degradation of emerging contaminants

  • 期刊论文

  • 发表日期：

    2026年7月10日

查看全文
  • Jatinder Singh Randhawa,
  • Harmanpreet Meehnian

摘要

Emerging contaminants (ECs), including endocrine-disrupting chemicals, dyes, pharmaceuticals, and pesticides, are increasingly released into the environment due to untreated industrial discharges, agricultural runoff, and the inefficiency of conventional treatment methods. Even at trace concentrations, their persistence necessitates effective remedial measures. Conventional physical and chemical methods are often inefficient, costly, generate secondary pollutants. To overcome these challenges, enzyme-based technologies have gained attention as green alternatives owing to their high catalytic efficiency, substrate specificity, and ability to operate under mild conditions without generating secondary pollution. Ligninolytic enzymes such as laccase (Lac), lignin peroxidase (LiP), manganese peroxidase (MnP), versatile peroxidase (VP), and horseradish peroxidase (HRP), primarily derived from white-rot fungi, are promising biocatalyst for EC degradation due to their broad substrate specificity, high catalytic efficiency, and stability. However, poor operational stability, denaturation susceptibility, high production cost (USD 200–1000 per kg), and poor reusability (1–3 cycles) constrain the application of free enzymes. Consequently, enzyme immobilization has arisen as an effective strategy to overcome these problems. Nanoengineered supports for enzyme immobilization offer advantages such as high surface area, easy recovery, improved catalytic stability, and enzyme loading, reduced enzyme leaching, and easy magnetic separation. Nanoimmobilized ligninolytic enzymes exhibit 2–fivefold higher stability, retain 70–90% activity after 3–10 cycles, and achieve a 40–70% improvement in process economics. This review discusses recent advances in nanoengineered ligninolytic enzyme systems for EC remediation, emphasizing catalytic degradation mechanisms, immobilization strategies, enzyme-nanomaterial interactions, and factors influencing catalytic performance. It also highlights large-scale implementation of immobilized enzyme strategies for efficient EC removal and outlines future research directions.