<p>The escalating burden of wastewater pollution demands sustainable and effective treatment solutions. Bamboo biochar has emerged as a promising adsorbent due to its renewable nature and favourable physicochemical properties associated with its surface functional groups. However, its reuse is limited by declining performance over repeated detoxification cycles. This review introduces a novel epigenetic approach to enhance the regeneration and functional lifespan of bamboo biochar. It begins by detailing the synthesis, structural attributes, and mechanisms that underpin its pollutant adsorption capacity. The limitations of conventional regeneration methods-thermal, chemical, and biological are critically examined. To overcome these constraints, the paper proposes an epigenetically guided framework using tools such as CRISPR/dCas9 and methyltransferase-based modulation. These strategies aim to reprogram epigenetic pathways to boost both bamboo regeneration and biochar quality. Case studies and hypothetical application models in municipal and industrial wastewater contexts are explored. Environmental and economic evaluations further highlight the feasibility of this approach, taking into account life cycle impacts, safety, scalability, and regulatory considerations. The review concludes by outlining future research directions, including AI-assisted design, expansion to alternative biochar feedstocks, and the need for interdisciplinary collaboration. Collectively, this work positions epigenetic reprogramming as a transformative and regenerative strategy in biochar-based wastewater treatment.</p>