<p>Cable-stayed bridges are increasingly used in China’s bridge construction due to their advantages of large span and good load bearing. Meanwhile, with the continuous development of highway construction level, the expectations of traffic participants for the function of bridges are no longer limited to passage, but also need to take into account the comfort of passage. In order to explore the comfort of the vehicle through the cable-stayed bridge, the large-span cable-stayed bridge as a model, the vehicle and the bridge are divided into two independent systems, respectively, the establishment of power balance equations, based on the displacement and force balance conditions to establish the vehicle-bridge coupling equations, the use of ANSYS vehicle-bridge coupling procedure to calculate the vertical acceleration of the vehicle, and then based on the international standard ISO2631-1 to establish the Matlab evaluation procedure for the travelling comfort. Then the Matlab evaluation program was established to evaluate the driving comfort according to the international standard ISO2631-1, and the effects of vehicle weight, driving speed and bridge deck unevenness on driving comfort were analysed, in which the bridge deck unevenness was simulated by using the trigonometric series method.</p>