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<p>Natural sources such as bacteria have been reported to produce anticancer compounds. The potential role of lactic acid bacteria in cancer prevention and treatment is a major area of interest. The aim of this study was to assess the growth inhibitory effects and apoptosis inducing properties of <i>Lactobacillus casei</i> UT1 supernatant on K562 chronic myeloid leukemia cells. In this study, after bacterial culture and metabolite production, cells were treated with the bacterial supernatant, and MTT test was performed to assess cell viability. Apoptosis was evaluated using fluorescence microscopy, cell cycle analysis, and annexin V/PI staining. In addition, changes in relative expression of key genes, including <i>Survivin</i>, <i>Bcl2</i>, <i>p53</i>, and <i>Noxa</i> were analyzed using Real-Time PCR. The results of the study showed that 1) metabolites of <i>Lactobacillus casei</i> UT1 supernatant inhibit cell growth, induce the arrest of the S phase of the cell cycle, and induce apoptosis in K562 cells depending on time and concentration. 2) Treatment of K562 cells resulted in reduced expression of the <i>Survivin</i> and <i>Bcl2</i> genes, while simultaneously increasing the expression of both <i>p53</i> and <i>Noxa</i> genes. These findings confirm that metabolites of <i>Lactobacillus casei</i> can effectively inhibit the growth of cancer cels and highlight their potential as a promising candidate for cancer treatment.</p>

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Lactobacillus Casei UT1 Supernatant Induce Cell Cycle Arrest and Apoptosis in K562 Leukemia Cells by Down Regulating of Bcl-2 and up-Regulating of Noxa

  • 期刊论文

  • 发表日期：

    2025年11月18日

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  • Fateme Shamsaee,
  • Samaneh Mohammadpour,
  • Aida Bahrami,
  • Mohammadkazem Bahrami,
  • Morteza Kosari-Nasab,
  • Gholamreza Zarrini,
  • Majid Mahdavi

摘要

Natural sources such as bacteria have been reported to produce anticancer compounds. The potential role of lactic acid bacteria in cancer prevention and treatment is a major area of interest. The aim of this study was to assess the growth inhibitory effects and apoptosis inducing properties of Lactobacillus casei UT1 supernatant on K562 chronic myeloid leukemia cells. In this study, after bacterial culture and metabolite production, cells were treated with the bacterial supernatant, and MTT test was performed to assess cell viability. Apoptosis was evaluated using fluorescence microscopy, cell cycle analysis, and annexin V/PI staining. In addition, changes in relative expression of key genes, including Survivin, Bcl2, p53, and Noxa were analyzed using Real-Time PCR. The results of the study showed that 1) metabolites of Lactobacillus casei UT1 supernatant inhibit cell growth, induce the arrest of the S phase of the cell cycle, and induce apoptosis in K562 cells depending on time and concentration. 2) Treatment of K562 cells resulted in reduced expression of the Survivin and Bcl2 genes, while simultaneously increasing the expression of both p53 and Noxa genes. These findings confirm that metabolites of Lactobacillus casei can effectively inhibit the growth of cancer cels and highlight their potential as a promising candidate for cancer treatment.