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<p>Achieving high-resolution 4D imaging (XYZt) of larger areas is paramount for comprehensively characterizing engineered tissues and disease models. Yet the high cost and optical requirements of glass-bottom devices, which are essential for confocal microscopy, often hinder the use of such advanced imaging. This study presents an innovative method for crafting cost-effective microfluidic devices to overcome this challenge. Diverging from traditional soft lithography techniques, which require cleanroom facilities and costly materials, our approach harnesses fused deposition modeling (FDM) to fabricate glass-bottom polydimethylsiloxane (PDMS) devices that seamlessly incorporate a 0.17&#xa0;mm glass coverslip optimized for laser scanning confocal microscopy (LSCM). Using glass-embedded acrylonitrile butadiene styrene (ABS) templates, we achieve precise fabrication of spiral channels in PDMS, thereby substantially reducing associated costs, including installation, infrastructure, and maintenance. The resultant device boasts numerous functionalities, facilitating diverse applications such as cell culture, reagent mixing, morphology monitoring, and on-chip immunoassays. Moreover, we showcase its versatility by demonstrating its efficacy for 4D calcium imaging with a resonance scanner in an LSCM, using HMC3 (human microglial) and MCF-7 (human breast cancer) cell lines. Beyond its cost-effectiveness, this biochip platform is suitable for applications such as toxicity analysis, drug screening, and real-time monitoring. This advancement promises new avenues for comprehensive bioimaging research, offering affordable and accessible solutions for studying complex biological systems. By democratizing access to high-resolution imaging, our method paves the way for a deeper understanding and characterization of diverse biological phenomena. Furthermore, its cost-effectiveness and ease of fabrication hold promise for adoption across various research fields, empowering researchers to further explore biological processes.</p>

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Fabrication of cost-effective glass-bottom cell-culture device using fused deposition modeling: new avenue for bioimaging

  • 期刊论文

  • 发表日期：

    2026年7月14日

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  • Sarpras Swain,
  • Shahna Shahul Hameed,
  • Shubha Jain,
  • S. Surya Kumar,
  • Falguni Pati,
  • Harikrishnan Narayanan Unni,
  • Mrutyunjay Suar,
  • Lopamudra Giri

摘要

Achieving high-resolution 4D imaging (XYZt) of larger areas is paramount for comprehensively characterizing engineered tissues and disease models. Yet the high cost and optical requirements of glass-bottom devices, which are essential for confocal microscopy, often hinder the use of such advanced imaging. This study presents an innovative method for crafting cost-effective microfluidic devices to overcome this challenge. Diverging from traditional soft lithography techniques, which require cleanroom facilities and costly materials, our approach harnesses fused deposition modeling (FDM) to fabricate glass-bottom polydimethylsiloxane (PDMS) devices that seamlessly incorporate a 0.17 mm glass coverslip optimized for laser scanning confocal microscopy (LSCM). Using glass-embedded acrylonitrile butadiene styrene (ABS) templates, we achieve precise fabrication of spiral channels in PDMS, thereby substantially reducing associated costs, including installation, infrastructure, and maintenance. The resultant device boasts numerous functionalities, facilitating diverse applications such as cell culture, reagent mixing, morphology monitoring, and on-chip immunoassays. Moreover, we showcase its versatility by demonstrating its efficacy for 4D calcium imaging with a resonance scanner in an LSCM, using HMC3 (human microglial) and MCF-7 (human breast cancer) cell lines. Beyond its cost-effectiveness, this biochip platform is suitable for applications such as toxicity analysis, drug screening, and real-time monitoring. This advancement promises new avenues for comprehensive bioimaging research, offering affordable and accessible solutions for studying complex biological systems. By democratizing access to high-resolution imaging, our method paves the way for a deeper understanding and characterization of diverse biological phenomena. Furthermore, its cost-effectiveness and ease of fabrication hold promise for adoption across various research fields, empowering researchers to further explore biological processes.