<p>This review provides a comprehensive perspective on the synthesis of nanoparticles using 3D-printed microfluidic devices and their related applications. The work delves into the confluence of 3D printing and microfluidic technology, and how it could help researchers address inherent limitations in the design, cost, and fabrication time of microfluidic devices. The paper investigates the benefits of various 3D printing techniques (Material extrusion, Vat photopolymerization, and Material jetting) in the fabrication of microfluidic devices (Reactionware and Droplet-based) and presents novel applications of such devices related to separation and manipulation of biological samples. Techniques for manufacturing diverse nanoparticles are also examined, highlighting the crucial role of 3D printed microchannel designs in achieving precise and reliable high-throughput applications. The applications of these nanoparticles range from drug delivery and encapsulation to electrode modification and optical enhancement. 3D printed microfluidic devices are revolutionizing nanoparticle fabrication with enhanced throughput and controlled particle sizes, suggesting a promising future for this technology.</p>