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Background <p>The possible involvement of human papillomavirus (HPV) in breast cancer remains a subject of continuous discussion and research. Some studies have identified HPV DNA in breast cancer samples, whereas others have not demonstrated a definitive correlation. This study sought to investigate the prevalence of HPV in breast cancer tissue samples from patients in Ardabil, Iran, to enhance the understanding of this potential association.</p> Methods <p>A total of 100 tissue samples were evaluated, consisting of 50 breast cancer samples and 50 healthy control samples. Fresh-frozen specimens were transported and stored correctly until processing. DNA extraction was conducted via a commercial kit, and specific primers targeting the HPV L1 gene were utilized for PCR amplification. Conventional PCR, agarose gel electrophoresis, and real-time PCR were employed for HPV identification.</p> Results <p>Despite utilizing highly sensitive PCR methodologies, including real-time PCR, no HPV DNA sequences were identified in any studied breast cancer tissue samples.</p> Conclusion <p>The involvement of HPV in breast carcinogenesis is still under investigation and contentious, with recent studies yielding conflicting data. However, this study found no HPV DNA in breast cancer samples from Ardabil, Iran.</p>

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Investigation of Human Papillomavirus Types in Breast Cancer Samples From Ardabil Province, Iran

  • 期刊论文

  • 发表日期：

    2026年2月10日

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  • Shayan Hamdollahzadeh,
  • Iraj Feizi,
  • Peyman Azghani,
  • Shahnaz Hosseinzadeh,
  • Chiman Karami

摘要

Background

The possible involvement of human papillomavirus (HPV) in breast cancer remains a subject of continuous discussion and research. Some studies have identified HPV DNA in breast cancer samples, whereas others have not demonstrated a definitive correlation. This study sought to investigate the prevalence of HPV in breast cancer tissue samples from patients in Ardabil, Iran, to enhance the understanding of this potential association.

Methods

A total of 100 tissue samples were evaluated, consisting of 50 breast cancer samples and 50 healthy control samples. Fresh-frozen specimens were transported and stored correctly until processing. DNA extraction was conducted via a commercial kit, and specific primers targeting the HPV L1 gene were utilized for PCR amplification. Conventional PCR, agarose gel electrophoresis, and real-time PCR were employed for HPV identification.

Results

Despite utilizing highly sensitive PCR methodologies, including real-time PCR, no HPV DNA sequences were identified in any studied breast cancer tissue samples.

Conclusion

The involvement of HPV in breast carcinogenesis is still under investigation and contentious, with recent studies yielding conflicting data. However, this study found no HPV DNA in breast cancer samples from Ardabil, Iran.