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Background <p>Natural flavonoids such as fisetin have gained attention for their potential anticancer properties. This study evaluates the cytotoxic and apoptotic effects of fisetin on MCF-7 human breast cancer cells.</p> Objective <p>To assess the dose-dependent cytotoxicity, apoptotic potential, and molecular interactions of fisetin with key apoptotic proteins.</p> Methods <p>MCF-7 cells were treated with increasing concentrations of fisetin, and cell viability was determined. Molecular docking was performed to analyze fisetin’s interactions with FOXO1, p53, and BCL2. Morphological changes in treated cells were examined microscopically.</p> Results <p>Fisetin exhibited dose-dependent cytotoxic effects on MCF-7 cells. Docking analysis showed strong interactions with apoptotic proteins, suggesting a role in apoptosis regulation. Microscopic observations confirmed morphological changes indicative of apoptosis.</p> Conclusion <p>Fisetin exhibits preliminary cytotoxic and morphological effects suggestive of apoptosis in MCF-7 cells, supported by molecular docking predictions. Further mechanistic studies are warranted to confirm apoptotic pathways and therapeutic relevance.</p>

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Cytotoxic and Apoptotic Effects of Fisetin on MCF-7 Breast Cancer Cells: A Molecular Docking and Morphological Study

  • 期刊论文

  • 发表日期：

    2025年10月14日

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  • Tharansia Ramachandran,
  • Gayathri Rengasamy,
  • J. Selvaraj,
  • V. Vishnu Priya

摘要

Background

Natural flavonoids such as fisetin have gained attention for their potential anticancer properties. This study evaluates the cytotoxic and apoptotic effects of fisetin on MCF-7 human breast cancer cells.

Objective

To assess the dose-dependent cytotoxicity, apoptotic potential, and molecular interactions of fisetin with key apoptotic proteins.

Methods

MCF-7 cells were treated with increasing concentrations of fisetin, and cell viability was determined. Molecular docking was performed to analyze fisetin’s interactions with FOXO1, p53, and BCL2. Morphological changes in treated cells were examined microscopically.

Results

Fisetin exhibited dose-dependent cytotoxic effects on MCF-7 cells. Docking analysis showed strong interactions with apoptotic proteins, suggesting a role in apoptosis regulation. Microscopic observations confirmed morphological changes indicative of apoptosis.

Conclusion

Fisetin exhibits preliminary cytotoxic and morphological effects suggestive of apoptosis in MCF-7 cells, supported by molecular docking predictions. Further mechanistic studies are warranted to confirm apoptotic pathways and therapeutic relevance.