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Introduction <p>Triple-negative breast cancer (TNBC) is one of the resistant types of breast cancer, and chemotherapy is the only systemic therapy for TNBC patients. Most patients have been treated with a type of chemotherapy drug, doxorubicin (DOX), which is an anthracycline family, and its major adverse effect is cardiotoxicity, which may limit its use.</p> Methods <p>This study investigated the effect of combination therapy, including selenium and low-level laser irradiation, on increasing the effect of DOX on MDA-MB-231 breast cancer cells. For this purpose, after culturing MDAMB- 231 cells, these cells were treated with different concentrations of DOX alone, selenium alone, and DOX and selenium combination in the presence and absence of low-level laser irradiation. The cell viability was measured by MTT assay, and then morphological changes of cells were examined by light microscopy. The rate of apoptotic cell death was examined by fluorescence microscopy and flow cytometry assay. The amount of ROS was measured via flow cytometry assay after treatment.</p> Results <p>The results show that DOX in combination with selenium in the presence of low-level laser irradiation can induce more cell death and ROS than DOX alone.</p> Conclusion <p>The use of selenium in combination with DOX in the presence of low-level laser irradiation by reducing the dose of DOX and at the same time increasing its effect can be recommended as a therapeutic strategy to overcome the side effects of DOX.</p>

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Translational Approach: Dual-Mode Therapy with Selenium and Irradiation Enhances Doxorubicin Effectiveness in Triple-Negative Breast Cancer

  • 期刊论文

  • 发表日期：

    2025年4月12日

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  • Mahdiyeh Samadi Ghane,
  • Zahra Kianmehr,
  • Khatereh Khorsandi,
  • Fedora Khatibi Shahidi

摘要

Introduction

Triple-negative breast cancer (TNBC) is one of the resistant types of breast cancer, and chemotherapy is the only systemic therapy for TNBC patients. Most patients have been treated with a type of chemotherapy drug, doxorubicin (DOX), which is an anthracycline family, and its major adverse effect is cardiotoxicity, which may limit its use.

Methods

This study investigated the effect of combination therapy, including selenium and low-level laser irradiation, on increasing the effect of DOX on MDA-MB-231 breast cancer cells. For this purpose, after culturing MDAMB- 231 cells, these cells were treated with different concentrations of DOX alone, selenium alone, and DOX and selenium combination in the presence and absence of low-level laser irradiation. The cell viability was measured by MTT assay, and then morphological changes of cells were examined by light microscopy. The rate of apoptotic cell death was examined by fluorescence microscopy and flow cytometry assay. The amount of ROS was measured via flow cytometry assay after treatment.

Results

The results show that DOX in combination with selenium in the presence of low-level laser irradiation can induce more cell death and ROS than DOX alone.

Conclusion

The use of selenium in combination with DOX in the presence of low-level laser irradiation by reducing the dose of DOX and at the same time increasing its effect can be recommended as a therapeutic strategy to overcome the side effects of DOX.