Abstract <p><i>Artocarpus (A.) heterophyllus</i> is a member of the Moraceae family, all parts of which contain sticky white latex produced by secretory cells called laticifers. <i>A. heterophyllus</i> latex was collected from the fresh and mature fruit, washed, and dried under vacuum to extract the resin. The resin obtained from the latex of <i>A. heterophyllus</i> may have the potential to be used as filler and additive restorative material in dentistry. The compounds present in the resin obtained from the latex were analysed by the GC-MS technique. The aim of this study is to extract the resin from the latex of <i>A. heterophyllus</i> using a convenient method and analyse the unknown compounds present in it by using GC-MS (Gas Chromatography-Mass Spectrometry) analysis and the present compounds to be obtained in resin are such as 9,19-Cyclocholan-24-al, 3-(acetyloxy)-4,4,14-trimethyl, 4,4-Dimethyl-cholesta-8,24-diene-1TMS, [(3β)-lanosta-8,24-dien-3-yl]oxy-1TMS and Trimethylsiloxycycloartenol-1TMS. To check out the structural, morphological properties and strength of the dental filling material, the XRD, SEM followed by EDAX, compressive and three-point bending were performed. After the filling of material into the teeth, the GC-MS was done and a solubility test was performed with various solvents of the dental filling material. Further, to check the cytotoxicity of the material the MTT assay was also performed. Antimicrobial property of the extracted resin was tested against gram positive strain <i>S. epidermidis</i> and <i>S. mutans.</i> Changing growth prospects in the scientific and medical domain may have a widespread and great prospect for these innovative compounds and their applications.</p> Lay Summary <p>The current research article focused on the extraction of the purified white latex resin from the jackfruit (<i>Artocarpus heterophyllus</i>) for dental filling application. As the dental fillings must withstand the pressure exerted while chewing, the collected resin possesses the high compressive strength and durability along with biocompatibility. Moreover, the extracted resin holds a promising position as a filler material in the dental restoration, which is explored by a sophisticated technique GC-MS. Overall, the combination of high mechanical strength, biocompatibility, and antimicrobial activity, significantly suggests that this natural resin could become a valuable resource in the development of eco-friendly dental materials, opening the door to innovative applications in dentistry and beyond.</p>