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Context <p>The high incidence of skin injuries caused by mechanical trauma, burns, diabetes, aging, bedsores, surgery, and various chronic diseases indicates the need for new approaches in skin regeneration. Decellularized biological scaffolds such as amniotic membrane, dermis, and pericardium have achieved acceptable results and a bright future in this field. Ostrich pericardium is a strong, thin, and tear-resistant tissue (compared to previous sources), but its potential for use in skin tissue engineering has not yet been evaluated.</p> Methods <p>In this study, ostrich pericardium was isolated and decellularized with two detergents, SDS and SD, and the efficiency of the decellularization method and its effect on the pericardium structure for use in skin tissue engineering were evaluated by DNA content analysis, H&amp;E, TC, and PAS staining, SEM, MTT assay, FTIR, tensile test, contact angle, water absorption, hemocompatibility, and blood coagulation index.</p> Results <p>The results showed that both methods reduced DNA content by 96.66% (without significant difference from each other), but the SD method was better able to preserve the ECM structure and collagen fibril arrangement. SEM results also confirmed the histological results by confirming the effective removal of cells and showed negligible structural destruction in the SDS method. Mechanical evaluations also confirmed the destructive effect of SDS in reducing the tensile strength of the final construct to 41.66% of native ostrich pericardium, while the SD method had the highest tensile strength (12.76&#xa0;MPa) with a slight increase in strength compared to native pericardium. The MTT test results did not show significant toxicity in either group. A 1.4-fold increase in water absorption, 1.66-fold increase in hydrophilicity, and better cell attachment to the surface of the final scaffold were other evidence that suggested the SD method as the preferred method for decellularization of ostrich pericardium.</p> Conclusions <p>Overall, our results showed that SD-decellularized ostrich pericardium (as an available and disposable tissue) has a high potential and a bright future for skin tissue engineering and accelerated skin defect regeneration.</p> Lay Summary <p>Biocompatible decellularized scaffolds play an important role in tissue regeneration. This study decellularized ostrich pericardial tissue using two different methods and evaluated its nature as a suitable scaffold for skin regeneration. Decellularization using the SD method better preserved the extracellular matrix, highlighting its favorable potential for tissue engineering applications.</p>

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Evaluation of Decellularized Ostrich Pericardium Using SDS or SD for in Vitro Tissue Reconstruction

  • 期刊论文

  • 发表日期：

    2026年2月25日

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  • Maliheh Gharibshahian,
  • Mozafar Khazaei,
  • Azam Bozorgi Zarrini,
  • Elham Ghanbari,
  • Mohammad Rasool Khazaei,
  • Mohammed Wali Mustafa,
  • Mustafa Imad Mohammed,
  • Leila Rezakhani

摘要

Context

The high incidence of skin injuries caused by mechanical trauma, burns, diabetes, aging, bedsores, surgery, and various chronic diseases indicates the need for new approaches in skin regeneration. Decellularized biological scaffolds such as amniotic membrane, dermis, and pericardium have achieved acceptable results and a bright future in this field. Ostrich pericardium is a strong, thin, and tear-resistant tissue (compared to previous sources), but its potential for use in skin tissue engineering has not yet been evaluated.

Methods

In this study, ostrich pericardium was isolated and decellularized with two detergents, SDS and SD, and the efficiency of the decellularization method and its effect on the pericardium structure for use in skin tissue engineering were evaluated by DNA content analysis, H&E, TC, and PAS staining, SEM, MTT assay, FTIR, tensile test, contact angle, water absorption, hemocompatibility, and blood coagulation index.

Results

The results showed that both methods reduced DNA content by 96.66% (without significant difference from each other), but the SD method was better able to preserve the ECM structure and collagen fibril arrangement. SEM results also confirmed the histological results by confirming the effective removal of cells and showed negligible structural destruction in the SDS method. Mechanical evaluations also confirmed the destructive effect of SDS in reducing the tensile strength of the final construct to 41.66% of native ostrich pericardium, while the SD method had the highest tensile strength (12.76 MPa) with a slight increase in strength compared to native pericardium. The MTT test results did not show significant toxicity in either group. A 1.4-fold increase in water absorption, 1.66-fold increase in hydrophilicity, and better cell attachment to the surface of the final scaffold were other evidence that suggested the SD method as the preferred method for decellularization of ostrich pericardium.

Conclusions

Overall, our results showed that SD-decellularized ostrich pericardium (as an available and disposable tissue) has a high potential and a bright future for skin tissue engineering and accelerated skin defect regeneration.

Lay Summary

Biocompatible decellularized scaffolds play an important role in tissue regeneration. This study decellularized ostrich pericardial tissue using two different methods and evaluated its nature as a suitable scaffold for skin regeneration. Decellularization using the SD method better preserved the extracellular matrix, highlighting its favorable potential for tissue engineering applications.