Abstract <p>Organoids have emerged as a groundbreaking innovation in dentistry, offering three-dimensional, self-organizing structures derived from stem cells that mimic the intricate architecture and functionality of native tissues. These advanced models effectively replicate complex biological interactions and pathological conditions, addressing the limitations of traditional two-dimensional cell cultures and animal models. Notably, organoids have demonstrated their potential in modeling various craniofacial tissues, including dental pulp, periodontal structures, and salivary glands. However, despite these significant advancements, the full integration of organoid technology into dental research and clinical practice remains an ongoing challenge due to issues related to scalability, standardization, and adaptation to existing treatment approaches. This review consolidates recent progress in organoid technology, emphasizing its implications for dental research and clinical applications. Incorporating organoid-based systems holds the promise of advancing the understanding of oral diseases, refining therapeutic strategies, and enabling patient-specific treatment approaches, particularly for conditions such as salivary gland dysfunction, dental anomalies, and oral tumors. Additionally, organoids play a pivotal role in regenerative dentistry, personalized medicine, and drug testing, showcasing their potential to revolutionize dental care. Future applications may extend to pediatric orthodontics and the bioengineering of dental tissues, leveraging the body’s innate regenerative abilities to enhance patient outcomes. By re-examining the potential of organoids as innovative tools in dental science, this manuscript explores their ability to facilitate more effective and individualized approaches to oral health management. Addressing current limitations while exploring new frontiers, organoid technology stands at the forefront of transforming dental research and clinical practice, paving the way for personalized, regenerative, and highly effective oral healthcare solutions.</p> Lay Summary <p>Organoids represent diminutive, laboratory-cultivated entities that emulate authentic human tissues and organs. These structures are derived from stem cells, which can proliferate and autonomously organize into three-dimensional architectures like those within the human body. In contrast to conventional planar (2D) cell cultures, organoids are adept at simulating intricate biological processes, rendering them invaluable for investigating the mechanisms underlying organ development and functionality. In the field of dentistry, organoids are emerging as a transformative technology. Conventional dental research predominantly depends on animal models or simplistic laboratory cultures, failing to adequately encompass the complexities inherent in human dental tissues. Organoids bridge this divide by accurately replicating the architecture and functionality of teeth, salivary glands, and jawbones. Such advancements can potentially revolutionize therapeutic approaches for oral diseases, dental anomalies, and even specific forms of oral malignancies. Regenerative dentistry is one of the most exciting uses for organoids. Dentists might employ organoids to develop replacement tissues rather than only implants or grafts, aiding patients with missing teeth, periodontal disease, or injured salivary glands. Additionally, organoids reduce the need for animal testing by providing a more accurate and humane method of testing new medications. Organoid technology may eventually result in customised dental care, where the patient's cells are utilised to produce unique treatments. For people with severe dental problems or uncommon disorders, this could change their lives. Organoids have the potential to revolutionise dentistry by improving the accuracy, efficacy, and patient experience of treatments, but further study is required.</p>