Scholarlink

Background <p>Recent advancements in bone tissue engineering emphasize the development of composite scaffolds integrated with phytochemicals to enhance bone repair through estrogenic activity, oxidative stress reduction, anti-inflammatory effects, and regulation of bone-signaling pathways. This study focused on synthesizing, characterizing, and evaluating Gelatin/Hydroxyapatite scaffolds incorporated with <i>Diosgenin</i>, a naturally occurring steroidal sapogenin with therapeutic potential in bone regeneration.</p> Methods <p>Electrospinning was employed to fabricate porous Gelatin/Hydroxyapatite/<i>Diosgenin</i> scaffolds. Their morphology and crystallinity were analyzed using Fourier Transform Infrared Spectroscopy, Scanning Electron Microscopy, and X-Ray Diffraction. Thermal stability was evaluated via Thermogravimetric Analysis. The osteogenic potential of <i>Diosgenin</i> was examined through in silico studies, while cytocompatibility was assessed using the MTT assay on MG63 osteosarcoma cell lines. Developmental toxicity was evaluated by exposing zebrafish embryos to concentrations ranging from 1.56 to 100&#xa0;µg/µL at 24, 48, 72, and 96&#xa0;h post fertilization.</p> Results <p>The electrospun scaffolds exhibited well-defined porous morphology, crystalline integrity, and favorable thermal stability. In vitro studies demonstrated significant cell proliferation and viability in MG63 cells, indicating cytocompatibility of the composite. Zebrafish embryo assays confirmed the safety profile of the <i>Diosgenin</i>-based scaffolds within the tested concentration range.</p> Conclusion <p>Gelatin/Hydroxyapatite/<i>Diosgenin</i> scaffolds exhibit excellent physicochemical stability, biocompatibility, and osteoinductive potential. These findings underscore their promise as effective biomaterials for bone tissue engineering and regenerative medicine applications.</p> Lay Summary with Future Directions <p> Bone injuries often need advanced treatments beyond natural healing. In this study, we developed a scaffold made of Gelatin and Hydroxyapatite, enriched with <i>Diosgenin</i>, a plant compound known for bone-strengthening and anti-inflammatory effects. The scaffold was fabricated using electrospinning and tested for structure, stability, and safety. Laboratory studies confirmed that it supported bone cell growth, while <i>in-silico</i> studies highlighted its role in bone-regenerating pathways. Zebrafish embryo tests showed that low diosgenin levels were safe for development. These findings suggest that <i>Diosgenin</i>-based scaffolds are promising for bone repair and regeneration. Future work will focus on optimizing <i>Diosgenin</i> dosage, validating long-term effects in animal models, and exploring clinical applications to advance this scaffold as a potential therapy in regenerative medicine.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Evaluation of Diosgenin-Enhanced Gelatin/Hydroxyapatite Biomaterial for Bone Regeneration Using in Silico, in Vitro, and Zebrafish Models

  • 期刊论文

  • 发表日期：

    2025年12月16日

查看全文
  • Janani Balasundaram Maragathavalli,
  • Saranya Srinivasan,
  • Ashwathi Vijayalekha,
  • Jayendran Udaiappan,
  • Gobi Saravanan Kaliaraj,
  • Suresh Kumar Anandasadagopan,
  • Ashok Kumar Pandurangan

摘要

Background

Recent advancements in bone tissue engineering emphasize the development of composite scaffolds integrated with phytochemicals to enhance bone repair through estrogenic activity, oxidative stress reduction, anti-inflammatory effects, and regulation of bone-signaling pathways. This study focused on synthesizing, characterizing, and evaluating Gelatin/Hydroxyapatite scaffolds incorporated with Diosgenin, a naturally occurring steroidal sapogenin with therapeutic potential in bone regeneration.

Methods

Electrospinning was employed to fabricate porous Gelatin/Hydroxyapatite/Diosgenin scaffolds. Their morphology and crystallinity were analyzed using Fourier Transform Infrared Spectroscopy, Scanning Electron Microscopy, and X-Ray Diffraction. Thermal stability was evaluated via Thermogravimetric Analysis. The osteogenic potential of Diosgenin was examined through in silico studies, while cytocompatibility was assessed using the MTT assay on MG63 osteosarcoma cell lines. Developmental toxicity was evaluated by exposing zebrafish embryos to concentrations ranging from 1.56 to 100 µg/µL at 24, 48, 72, and 96 h post fertilization.

Results

The electrospun scaffolds exhibited well-defined porous morphology, crystalline integrity, and favorable thermal stability. In vitro studies demonstrated significant cell proliferation and viability in MG63 cells, indicating cytocompatibility of the composite. Zebrafish embryo assays confirmed the safety profile of the Diosgenin-based scaffolds within the tested concentration range.

Conclusion

Gelatin/Hydroxyapatite/Diosgenin scaffolds exhibit excellent physicochemical stability, biocompatibility, and osteoinductive potential. These findings underscore their promise as effective biomaterials for bone tissue engineering and regenerative medicine applications.

Lay Summary with Future Directions

Bone injuries often need advanced treatments beyond natural healing. In this study, we developed a scaffold made of Gelatin and Hydroxyapatite, enriched with Diosgenin, a plant compound known for bone-strengthening and anti-inflammatory effects. The scaffold was fabricated using electrospinning and tested for structure, stability, and safety. Laboratory studies confirmed that it supported bone cell growth, while in-silico studies highlighted its role in bone-regenerating pathways. Zebrafish embryo tests showed that low diosgenin levels were safe for development. These findings suggest that Diosgenin-based scaffolds are promising for bone repair and regeneration. Future work will focus on optimizing Diosgenin dosage, validating long-term effects in animal models, and exploring clinical applications to advance this scaffold as a potential therapy in regenerative medicine.