Purpose <p>This review explores the potential of tissue adhesives as an effective and less invasive alternative to traditional wound closure techniques such as sutures and staples. The primary research question addresses the classification, mechanisms, and functional properties of tissue adhesives that contribute to their adhesion strength, healing efficiency, and biomedical applications.</p> Methods <p>The review categorizes tissue adhesives into synthetic, natural, and biomimetic types, analyzing their roles in adhesion, hemostasis, sealing, and microbial barrier formation. It further examines the critical functional groups—hydroxyl, carboxyl, amine, and carbonyl—present in both tissue adhesives and human tissues, emphasizing their role in bond formation. A discussion on the kinetics of adhesion and functional group interactions is included to highlight the significance of these chemical properties in adhesive performance.</p> Results <p>Tissue adhesives offer a user-friendly application, enhance healing speed, reduce trauma during wound closure, enable localized drug delivery, and provide flexibility. Their adhesive strength depends significantly on the specific functional groups involved in binding with human tissues. The mechanical strength of the bonds can be modulated by adjusting the biomaterial composition, optimizing tissue adhesion for various medical applications.</p> Conclusion <p>Tissue adhesives serve as a promising alternative for wound closure, providing rapid and painless healing with additional functionalities such as sealing, hemostasis, and antimicrobial protection. The effectiveness of these adhesives is largely influenced by their chemical composition, particularly the functional groups involved in adhesion. Understanding the interactions between these groups and their kinetics is crucial for advancing tissue adhesive technology and improving clinical outcomes.</p> Graphical Abstract <p></p>