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Purpose <p>To investigate the efficacy of a clinically correlated dose of topical sterile lyophilized amniotic membrane extract (AME) in a cutaneous wound model.</p> Methods <p>The study included 16 Wistar-Albino rats. A full-thickness skin defect was created on the dorsal surface of all rats. In group 1 (<i>n</i>=6), amniotic membrane transplantation (AMT) was performed. In group 2 (<i>n</i>=6), AME with an equivalent total protein ratio to that of the AMT was applied topically. In group 3 (<i>n</i>=4), the wound was covered with petroleum gauze containing chlorhexidine. Cutaneous wound healing was evaluated clinically and histopathologically.</p> Results <p>Macroscopically, smoother and thinner crust formation was present in group 1 compared to the other two groups. A more irregular wound surface and greater crust thickness were observed in group 3 compared to group 2. Histopathologically, curst formation was thinner and more regular in group 1. There was no statistically significant difference among groups in terms of vascularization, fibrosis, edema, ulceration, bacterial colonization, and inflammatory cell infiltration density scores.</p> Conclusion <p>Sterile lyophilized AME is clinically and histopathologically superior to the control group in cutaneous wound healing. Furthermore, based on these findings, while the efficacy of AMT in routine clinical use for wound healing is undeniable, AME also stands out for its ease of use.</p> <p>Lay Summary</p> <p>This study demonstrates the effects of topically applied AME on cutaneous wound healing both clinically and histopathologically, thereby representing a significant step towards the conversion of AME into a product for medical use in clinical practice. Topically applied AME has similar therapeutic efficacy to AMT in a cutaneous wound model, but AME is more advantageous in terms of storage and ease of use. AME has the potential as an alternative in reducing morbidity associated with chronic wounds, particularly those linked with infection and inflammation.</p>

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Clinically Correlated Dose-Use of Amniotic Membrane Extract in the Experimental Skin Wound Model

  • 期刊论文

  • 发表日期：

    2025年5月29日

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  • Nargiz Ibrahimli,
  • Ilayda Korkmaz,
  • Meltem Kocamanoglu,
  • Mehmet Gurdal,
  • Mesut Arici,
  • Banu Yaman,
  • Ozlem Barut Selver,
  • Ahmet Bicer

摘要

Purpose

To investigate the efficacy of a clinically correlated dose of topical sterile lyophilized amniotic membrane extract (AME) in a cutaneous wound model.

Methods

The study included 16 Wistar-Albino rats. A full-thickness skin defect was created on the dorsal surface of all rats. In group 1 (n=6), amniotic membrane transplantation (AMT) was performed. In group 2 (n=6), AME with an equivalent total protein ratio to that of the AMT was applied topically. In group 3 (n=4), the wound was covered with petroleum gauze containing chlorhexidine. Cutaneous wound healing was evaluated clinically and histopathologically.

Results

Macroscopically, smoother and thinner crust formation was present in group 1 compared to the other two groups. A more irregular wound surface and greater crust thickness were observed in group 3 compared to group 2. Histopathologically, curst formation was thinner and more regular in group 1. There was no statistically significant difference among groups in terms of vascularization, fibrosis, edema, ulceration, bacterial colonization, and inflammatory cell infiltration density scores.

Conclusion

Sterile lyophilized AME is clinically and histopathologically superior to the control group in cutaneous wound healing. Furthermore, based on these findings, while the efficacy of AMT in routine clinical use for wound healing is undeniable, AME also stands out for its ease of use.

Lay Summary

This study demonstrates the effects of topically applied AME on cutaneous wound healing both clinically and histopathologically, thereby representing a significant step towards the conversion of AME into a product for medical use in clinical practice. Topically applied AME has similar therapeutic efficacy to AMT in a cutaneous wound model, but AME is more advantageous in terms of storage and ease of use. AME has the potential as an alternative in reducing morbidity associated with chronic wounds, particularly those linked with infection and inflammation.