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Purpose <p>Burn injuries with life-threatening effects are difficult to treat with traditional and common dressings. This study was aimed to develop silk sericin–based injectable hydrogels containing ethanolic extract of <i>Withania coagulans</i> for the treatment of burn wounds in mice.</p> Methods <p>A hot metal rod (6&#xa0;mm in diameter) was used to create burn wounds on the dorsal side of mice (<i>n</i> = 45) in all study groups (<i>n</i> = 9). Hydrogels were prepared and characterized, and various formulations of injectable hydrogels were injected around the periphery of the wounds in treatment groups. The regenerative potential of hydrogels was assessed by measuring wound healing time, percent wound contraction, and conducting histological analysis. Additionally, serum levels of various biochemical parameters such as pro-inflammatory cytokines including tumor necrosis factor-α (TNF-α) and Interleukin-6 (IL-6) as well as the anti-inflammatory cytokine IL-10, tissue inhibitor of metalloproteinases (TIMPs), and matrix metalloproteinases (MMP-2, MMP-9) were measured.</p> Results <p>The pH values of all hydrogels were between 3.0 and 4.0, with viscosity ranging from 62 to 105 cSt at 40&#xa0;°C, confirming their injectability. All the mice given injectable hydrogels showed significantly faster wound healing (<i>P</i> &lt; 0.05) as compared to negative control, which showed only 43% reduction in wound diameter at post injury day 17. However, the percentage wound contraction was highest (83%) in T7 group (4% silk sericin and 2% <i>W. coagulans</i>) as compared to other treatment groups (50–67%). Similarly, all the hydrogel treated groups showed significantly higher levels of IL-10 and TIMPs and significantly lower levels of TNF-α, IL-6, and MMP-2 and MMP-9as compared to negative control. Moreover, among all the treatment groups, the level of IL-6, IL-10, TNF-α, TIMPs, MMP-2, and MMP-9 in T7 was significantly different, further confirming its superior wound healing potentials.</p> Conclusion <p>In conclusion, silk sericin–based hydrogels were injectable and possess superior healing and regenerative properties against burn wounds. The hydrogel of 4% silk sericin and 2% <i>W. coagulans</i> was the most effective among all.</p> Lay Summary <p>Injectable hydrogels, with superior biological and physiochemical characteristics, allow the targeted delivery of drug to irregular shaped wound. Therefore, in this study, silk sericin–based injectable hydrogels infused with <i>W. coagulans</i> extract were prepared. It was observed that these hydrogels were injectable and possess superior burn wound healing efficacies.</p>

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Formulation and Evaluation of Silk Sericin and Xanthan Gum–Based Injectable Hydrogels for the Treatment of Burn Wound

  • 期刊论文

  • 发表日期：

    2025年3月13日

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  • Kiran Zainab,
  • Hafiz Muhammad Tahir,
  • Aamir Ali,
  • Shaukat Ali,
  • Ayesha Muzamil,
  • Fatima Ijaz,
  • Asjid Ghaffar,
  • Muhammad Tayyab Ali,
  • Fariha Munir

摘要

Purpose

Burn injuries with life-threatening effects are difficult to treat with traditional and common dressings. This study was aimed to develop silk sericin–based injectable hydrogels containing ethanolic extract of Withania coagulans for the treatment of burn wounds in mice.

Methods

A hot metal rod (6 mm in diameter) was used to create burn wounds on the dorsal side of mice (n = 45) in all study groups (n = 9). Hydrogels were prepared and characterized, and various formulations of injectable hydrogels were injected around the periphery of the wounds in treatment groups. The regenerative potential of hydrogels was assessed by measuring wound healing time, percent wound contraction, and conducting histological analysis. Additionally, serum levels of various biochemical parameters such as pro-inflammatory cytokines including tumor necrosis factor-α (TNF-α) and Interleukin-6 (IL-6) as well as the anti-inflammatory cytokine IL-10, tissue inhibitor of metalloproteinases (TIMPs), and matrix metalloproteinases (MMP-2, MMP-9) were measured.

Results

The pH values of all hydrogels were between 3.0 and 4.0, with viscosity ranging from 62 to 105 cSt at 40 °C, confirming their injectability. All the mice given injectable hydrogels showed significantly faster wound healing (P < 0.05) as compared to negative control, which showed only 43% reduction in wound diameter at post injury day 17. However, the percentage wound contraction was highest (83%) in T7 group (4% silk sericin and 2% W. coagulans) as compared to other treatment groups (50–67%). Similarly, all the hydrogel treated groups showed significantly higher levels of IL-10 and TIMPs and significantly lower levels of TNF-α, IL-6, and MMP-2 and MMP-9as compared to negative control. Moreover, among all the treatment groups, the level of IL-6, IL-10, TNF-α, TIMPs, MMP-2, and MMP-9 in T7 was significantly different, further confirming its superior wound healing potentials.

Conclusion

In conclusion, silk sericin–based hydrogels were injectable and possess superior healing and regenerative properties against burn wounds. The hydrogel of 4% silk sericin and 2% W. coagulans was the most effective among all.

Lay Summary

Injectable hydrogels, with superior biological and physiochemical characteristics, allow the targeted delivery of drug to irregular shaped wound. Therefore, in this study, silk sericin–based injectable hydrogels infused with W. coagulans extract were prepared. It was observed that these hydrogels were injectable and possess superior burn wound healing efficacies.