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<p>Coffee senna (<i>Senna occidentalis</i> L.) plants exhibiting mosaic, vein clearing and leaf distortion symptoms were observed in the roadsides in Palapye town, Central District of Botswana since 2022. Electron microscopic examination of a representative symptomatic sample showed the presence of flexuous filamentous particles resembling a potyvirus which could be trapped and decorated with antibodies raised against wisteria vein mosaic virus. Similar symptoms were observed in some locations in roadsides in northern towns of Letlhakane, Kavira, Kazungula and Nata in 2025. Upon mechanical inoculation of the Palapye isolate to several potential host species, only <i>Nicotiana benthamiana, N. clevelandi</i> and the original host (<i>S. occidentalis)</i> showed symptoms while the other non-legume and legume hosts did not, indicating a narrow host range of the putative virus. There was no evidence of seed transmission in <i>S. occidentalis</i>. High-throughput sequencing allowed the reconstruction of the almost complete genome sequence (9622 nt) of an unknown potyvirus encoding a predicted polyprotein of 3083 aa. Pairwise comparison of the polyprotein region sequences showed the highest identity (~73% and ~72% at aa and nt level, respectively) with East Asian Passiflora distortion virus (EAPDV), indicating the virus belongs to a new potyvirus species for which we suggest the name senna vein clearing virus (SVCV) and the tentative binomial species name <i>Potyvirus sennae</i>. Phylogenetically, SVCV is placed in a common bean mosaic virus group, EAPDV being its closest relative.</p>

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A novel potyvirus causing mosaic and vein clearing on coffee senna (Senna occidentalis L.)

  • 期刊论文

  • 发表日期：

    2026年1月12日

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  • Adane Abraham,
  • Dennis Knierim,
  • Paolo Margaria,
  • Wulf Menzel

摘要

Coffee senna (Senna occidentalis L.) plants exhibiting mosaic, vein clearing and leaf distortion symptoms were observed in the roadsides in Palapye town, Central District of Botswana since 2022. Electron microscopic examination of a representative symptomatic sample showed the presence of flexuous filamentous particles resembling a potyvirus which could be trapped and decorated with antibodies raised against wisteria vein mosaic virus. Similar symptoms were observed in some locations in roadsides in northern towns of Letlhakane, Kavira, Kazungula and Nata in 2025. Upon mechanical inoculation of the Palapye isolate to several potential host species, only Nicotiana benthamiana, N. clevelandi and the original host (S. occidentalis) showed symptoms while the other non-legume and legume hosts did not, indicating a narrow host range of the putative virus. There was no evidence of seed transmission in S. occidentalis. High-throughput sequencing allowed the reconstruction of the almost complete genome sequence (9622 nt) of an unknown potyvirus encoding a predicted polyprotein of 3083 aa. Pairwise comparison of the polyprotein region sequences showed the highest identity (~73% and ~72% at aa and nt level, respectively) with East Asian Passiflora distortion virus (EAPDV), indicating the virus belongs to a new potyvirus species for which we suggest the name senna vein clearing virus (SVCV) and the tentative binomial species name Potyvirus sennae. Phylogenetically, SVCV is placed in a common bean mosaic virus group, EAPDV being its closest relative.