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<p>The increasing use of pesticides in rice cultivation poses significant challenges to economic and ecological sustainability. Rice blast (RB) and bacterial blight (BB) diseases are responsible for substantial yield losses in Bangladesh. So, farmers are heavily relying on chemical pesticides to combat these diseases. This study aimed to stack RB (<i>Pb1</i>and <i>Pi9</i>) and BB (<i>Xa4</i>, <i>xa13</i>, and <i>Xa21</i>) resistant (R) genes in the genetic background of a popular high- yielding rice variety BRRI dhan92 through marker-assisted selection (MAS). For this, multiple crosses were performed and the generations from F<sub>1</sub> to BC<sub>3</sub>F<sub>5</sub> were advanced by constant backcrossing, selfing, and foreground selection. These R genes were introgressed from the donor parents Pb1-US2, Pi9-US2 for <i>Pb1</i> and <i>Pi9</i> respectively, and IRBB58 for <i>Xa4</i>, <i>xa13</i> and <i>Xa21</i>. A total of 25 advanced lines (ALs) possessing these R genes in various combinations were selected for further analysis. These ALs exhibited superior performance to BRRI dhan92 in all aspects. Fourteen ALs carried all five R genes, while nine lines contained four R genes. Low disease ratings (0 and 3 for RB and BB) were recorded from these ALs, whereas BRRI dhan92 exhibited a severe reaction (7 to 9). Several ALs like G2, G17, G1, G24, G10, G16, G6, and G22 demonstrated superior yield performance (8.97, 8.85, 8.84, 8.84, 8.83, 8.76, 8.75, and 8.85 tha<sup>-1</sup>respectively, compared to 8.51 tha<sup>-1</sup>for BRRI dhan92). These fixed ALs can be released as a blast and BB-resistant variety or may be used as a potential genetic stock to incorporate with other desirable genes to increase resistance or yield potentials.</p>

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Genetic enhancement of blast and bacterial blight resistance in the rice variety BRRI dhan92 through marker assisted breeding

  • 期刊论文

  • 发表日期：

    2025年9月18日

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  • Mohammad Abdul Latif,
  • Omar Kayess,
  • Lutfur Rahman,
  • Rakibul Hasan,
  • Mohammad Ashik Iqbal Khan

摘要

The increasing use of pesticides in rice cultivation poses significant challenges to economic and ecological sustainability. Rice blast (RB) and bacterial blight (BB) diseases are responsible for substantial yield losses in Bangladesh. So, farmers are heavily relying on chemical pesticides to combat these diseases. This study aimed to stack RB (Pb1and Pi9) and BB (Xa4, xa13, and Xa21) resistant (R) genes in the genetic background of a popular high- yielding rice variety BRRI dhan92 through marker-assisted selection (MAS). For this, multiple crosses were performed and the generations from F1 to BC3F5 were advanced by constant backcrossing, selfing, and foreground selection. These R genes were introgressed from the donor parents Pb1-US2, Pi9-US2 for Pb1 and Pi9 respectively, and IRBB58 for Xa4, xa13 and Xa21. A total of 25 advanced lines (ALs) possessing these R genes in various combinations were selected for further analysis. These ALs exhibited superior performance to BRRI dhan92 in all aspects. Fourteen ALs carried all five R genes, while nine lines contained four R genes. Low disease ratings (0 and 3 for RB and BB) were recorded from these ALs, whereas BRRI dhan92 exhibited a severe reaction (7 to 9). Several ALs like G2, G17, G1, G24, G10, G16, G6, and G22 demonstrated superior yield performance (8.97, 8.85, 8.84, 8.84, 8.83, 8.76, 8.75, and 8.85 tha-1respectively, compared to 8.51 tha-1for BRRI dhan92). These fixed ALs can be released as a blast and BB-resistant variety or may be used as a potential genetic stock to incorporate with other desirable genes to increase resistance or yield potentials.