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<p>Members of the <i>Ralstonia solanacearum</i> species complex (RSSC) cause wilt in a wide range of hosts and are among the most destructive pathogens in the world. Despite the importance of bacterial wilt in Musaceae and Solanaceae for the state of Alagoas in Brazil, there are still no studies that determined the distribution of species and variability of RSSC in this region. To fill this gap in knowledge, the distribution, abundance, sequence variant (sequevar) identity, and genetic diversity of <i>R. solanacearum</i> (phylotype II) and <i>R. pseudosolanacearum</i> (phylotype I) were evaluated using 102 bacterial isolates. These isolates were recovered from symptomatic tissues of banana, eggplant, pepper and tomato in the agreste and eastern mesoregions of the state of Alagoas, Brazil. Repetitive sequence-based PCR (rep-PCR) genotyping revealed 11 clonal lineages among the 102 isolates tested, of which 22 isolates were selected for partial sequencing of the endoglucanase (<i>egl</i>) gene. In the agreste mesoregion, only <i>R. solanacearum</i> sequevar IIA-6/35 was detected. The expected 220-bp amplification product that is specific for sequevar IIA-6 via Moko multiplex PCR (Mmx-PCR) was absent, despite the banana host was expressing vascular discoloration that is characteristic of banana Moko disease. <i>R. pseudosolanacearum</i> (sequevars I-17 and I-18) was more abundant than <i>R. solanacearum</i> (sequevars IIA-36, IIA-41 and IIA-53) in the eastern mesoregion, with percentages of 55.7% and 44.3%, respectively. These results provide new insights into RSSC sequevar and genetic diversity, species abundance and distribution in one of the main Musaceae and Solanaceae producing regions in northeast Brazil.</p>

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Ralstonia species infecting Solanaceae and banana occur at different relative abundances in distinct regions of Alagoas state, Brazil

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  • 发表日期：

    2025年7月14日

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  • Yolanda de Melo de Oliveira,
  • Lilia C. Carvalhais,
  • Iraildes Pereira Assunção,
  • Gaus Silvestre de Andrade Lima,
  • Samário Lino dos Santos,
  • Elineide Barbosa de Souza,
  • Wallison Oliveira Vieira,
  • Adriano Márcio Freire Silva

摘要

Members of the Ralstonia solanacearum species complex (RSSC) cause wilt in a wide range of hosts and are among the most destructive pathogens in the world. Despite the importance of bacterial wilt in Musaceae and Solanaceae for the state of Alagoas in Brazil, there are still no studies that determined the distribution of species and variability of RSSC in this region. To fill this gap in knowledge, the distribution, abundance, sequence variant (sequevar) identity, and genetic diversity of R. solanacearum (phylotype II) and R. pseudosolanacearum (phylotype I) were evaluated using 102 bacterial isolates. These isolates were recovered from symptomatic tissues of banana, eggplant, pepper and tomato in the agreste and eastern mesoregions of the state of Alagoas, Brazil. Repetitive sequence-based PCR (rep-PCR) genotyping revealed 11 clonal lineages among the 102 isolates tested, of which 22 isolates were selected for partial sequencing of the endoglucanase (egl) gene. In the agreste mesoregion, only R. solanacearum sequevar IIA-6/35 was detected. The expected 220-bp amplification product that is specific for sequevar IIA-6 via Moko multiplex PCR (Mmx-PCR) was absent, despite the banana host was expressing vascular discoloration that is characteristic of banana Moko disease. R. pseudosolanacearum (sequevars I-17 and I-18) was more abundant than R. solanacearum (sequevars IIA-36, IIA-41 and IIA-53) in the eastern mesoregion, with percentages of 55.7% and 44.3%, respectively. These results provide new insights into RSSC sequevar and genetic diversity, species abundance and distribution in one of the main Musaceae and Solanaceae producing regions in northeast Brazil.