Scholarlink

<p>Cassava bacterial blight (CBB) is the most limiting bacterial disease in the production of this crop. The growth of resistant varieties is the most efficient strategy for managing disease, even though the genetic bases of CBB resistance are not fully understood. Here we aim to identify allelic variants associated with CBB resistance in candidate genes <i>Manes.08G041100</i> and <i>Manes.13G039400</i>, which encode for defensin and subtilisin, respectively. The expression of these genes was evaluated in cassava genotypes with contrasting responses to inoculation by <i>Xanthomonas phaseoli</i> pv. manihotis (Xpm). <i>In vitro</i> phenotyping allowed us to describe the response to infection in 10 cassava genotypes. Despite no SNV-phenotype association from the statistical analysis, candidate gene expression was induced at 50 hpi, with differential expression of <i>Manes.08G041100</i> between contrasting genotypes. This study contributes for understanding the molecular basis of the <i>Xanthomonas</i>-cassava interaction and validates candidate CBB resistance genes.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Allelic variation and differential expression of cassava bacterial blight resistance candidate genes

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2025年7月25日

查看全文
  • Emili Ishikawa García,
  • Camilo Ernesto López Carrascal,
  • Johana Carolina Soto Sedano

摘要

Cassava bacterial blight (CBB) is the most limiting bacterial disease in the production of this crop. The growth of resistant varieties is the most efficient strategy for managing disease, even though the genetic bases of CBB resistance are not fully understood. Here we aim to identify allelic variants associated with CBB resistance in candidate genes Manes.08G041100 and Manes.13G039400, which encode for defensin and subtilisin, respectively. The expression of these genes was evaluated in cassava genotypes with contrasting responses to inoculation by Xanthomonas phaseoli pv. manihotis (Xpm). In vitro phenotyping allowed us to describe the response to infection in 10 cassava genotypes. Despite no SNV-phenotype association from the statistical analysis, candidate gene expression was induced at 50 hpi, with differential expression of Manes.08G041100 between contrasting genotypes. This study contributes for understanding the molecular basis of the Xanthomonas-cassava interaction and validates candidate CBB resistance genes.