Scholarlink

<p>RNA interference based antiviral defense generates small interfering RNAs.To gain insight into this mechanism during viral infections, we analyzed public small RNA sequencing (sRNA-seq) data and characterized the derived viral small RNA profiles from four monocotyledonous plant species. We retrieved public data from <i>Prostechea radiata</i><i>, </i><i>Tradescantia fluminensis</i><i>, </i><i>Zea mays</i>, and <i>Musa accuminata</i>, and annotated viral sequences that were previously undetected in those datasets. We reported for the first time the infection of cymbidium mosaic virus in <i>P. radiata</i> in addition to the presence of costus stripe mosaic virus in <i>T. fluminensis</i> and citrus exocortis viroid (CEVd) infection in <i>M. accuminata</i>; expanding the host range of these viruses. Afterwards, we characterized the viral-derived small RNA profile in these four plant-virus pathosystems. We showed differential abundance and the distribution of viral small interfering RNAs dominated by the 21-22 nucleotide class in most virus-host pathosystem, except for maize associated totivirus in <i>Z. mays</i> where the 22-24 nucleotide class was the most abundant. Moreover, we identified the key RNA silencing proteins involved in the antiviral response. These findings highlight the potential of RNA silencing as an effective control strategy against plant viruses and provide valuable insights into plant-virus interactions helping in the development of new control strategies to mitigate the impact of viral infections on important monocotyledonous crops.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Detection of viruses/viroid and characterization of their derived small RNAs in four monocotyledonous plants

  • 期刊论文

  • 发表日期：

    2025年7月14日

查看全文
  • Meryem Amara,
  • Latifa Berrada,
  • João Marcos Fagundes Silva,
  • Ayoub Maachi

摘要

RNA interference based antiviral defense generates small interfering RNAs.To gain insight into this mechanism during viral infections, we analyzed public small RNA sequencing (sRNA-seq) data and characterized the derived viral small RNA profiles from four monocotyledonous plant species. We retrieved public data from Prostechea radiata, Tradescantia fluminensis, Zea mays, and Musa accuminata, and annotated viral sequences that were previously undetected in those datasets. We reported for the first time the infection of cymbidium mosaic virus in P. radiata in addition to the presence of costus stripe mosaic virus in T. fluminensis and citrus exocortis viroid (CEVd) infection in M. accuminata; expanding the host range of these viruses. Afterwards, we characterized the viral-derived small RNA profile in these four plant-virus pathosystems. We showed differential abundance and the distribution of viral small interfering RNAs dominated by the 21-22 nucleotide class in most virus-host pathosystem, except for maize associated totivirus in Z. mays where the 22-24 nucleotide class was the most abundant. Moreover, we identified the key RNA silencing proteins involved in the antiviral response. These findings highlight the potential of RNA silencing as an effective control strategy against plant viruses and provide valuable insights into plant-virus interactions helping in the development of new control strategies to mitigate the impact of viral infections on important monocotyledonous crops.