Scholarlink

<p>Sheath blight (ShB), caused by&#xa0;<i>Rhizoctonia solani</i>, is a severe disease that poses a significant threat to global rice (<i>Oryza sativa</i> L.) production. This study employed next-generation sequencing-based bulk segregant analysis (BSA) combined with the R package QTLseqr to pinpoint genomic regions associated with sheath blight resistance. A total of 347 F<sub>3</sub> progenies, derived from a cross between the susceptible rice cultivar PR121 and the resistant donor ShB1 (IET22769), were analysed. From this F<sub>3</sub> population, fifteen moderately resistant and fifteen highly susceptible lines were selected to create ShB-R and ShB-S bulks, respectively. Using QTL-seq analysis with 1,145,820 high-quality single nucleotide polymorphisms (SNPs), five quantitative trait loci (QTLs) were identified: <i>qShB1</i>, <i>qShB3</i>, <i>qShB5</i>.1, <i>qShB5</i>.2, and <i>qShB6</i>, located on chromosomes 1, 3, 5, and 6 respectively. Notably, <i>qShB6</i>, spanning from 0.81 to 4.27&#xa0;Mb on chromosome 6, was identified as a novel QTL. Candidate gene analysis within these QTL regions revealed 69 putative genes, including those encoding leucine-rich repeat receptor-like kinases, coiled-coil nucleotide-binding proteins, and transcription factors, which may play a role in the defense mechanism against&#xa0;<i>R. solani</i>. This information can be leveraged to develop more effective disease management strategies. The identified QTLs and candidate genes provide valuable targets for molecular breeding efforts aimed at creating sheath blight-resistant rice varieties.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

QTL mapping and candidate gene identification for sheath blight resistance in rice (Oryza sativa) F3 progenies using QTL-seq analysis

  • 期刊论文

  • 发表日期：

    2025年7月25日

查看全文
  • Mayur R. Thesiya,
  • Jagjeet S. Lore,
  • Dharminder Bhatia,
  • Sanjay Kumar,
  • Mandeep S. Hunjan,
  • Jyoti Jain,
  • Rupinder Kaur

摘要

Sheath blight (ShB), caused by Rhizoctonia solani, is a severe disease that poses a significant threat to global rice (Oryza sativa L.) production. This study employed next-generation sequencing-based bulk segregant analysis (BSA) combined with the R package QTLseqr to pinpoint genomic regions associated with sheath blight resistance. A total of 347 F3 progenies, derived from a cross between the susceptible rice cultivar PR121 and the resistant donor ShB1 (IET22769), were analysed. From this F3 population, fifteen moderately resistant and fifteen highly susceptible lines were selected to create ShB-R and ShB-S bulks, respectively. Using QTL-seq analysis with 1,145,820 high-quality single nucleotide polymorphisms (SNPs), five quantitative trait loci (QTLs) were identified: qShB1, qShB3, qShB5.1, qShB5.2, and qShB6, located on chromosomes 1, 3, 5, and 6 respectively. Notably, qShB6, spanning from 0.81 to 4.27 Mb on chromosome 6, was identified as a novel QTL. Candidate gene analysis within these QTL regions revealed 69 putative genes, including those encoding leucine-rich repeat receptor-like kinases, coiled-coil nucleotide-binding proteins, and transcription factors, which may play a role in the defense mechanism against R. solani. This information can be leveraged to develop more effective disease management strategies. The identified QTLs and candidate genes provide valuable targets for molecular breeding efforts aimed at creating sheath blight-resistant rice varieties.