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<p>The aim of this research was to obtain protein-fractions from the aqueous extract of the fungus <i>P. sanguineus</i> through gel filtration chromatography (GFC) to induce resistance to <i>Meloidogyne</i> spp. in Micro-Tom tomato plants. Precipitation of protein from the aqueous extract (AE) of basidiocarps was performed based on increasing ethanol gradients 20%, 40%, 60%, 80% and 95%, which were individually centrifuged. Each ethanol-protein precipitate (EPP) was fractionated by using GFC, and isolated proteins (IP) were identified based on molecular mass. Six IP (named as I, II, III, IV, V and VI) were obtained through GFC, whose molecular weights were 4.81, 47.98, 0.17, 25.78, 4.25 and 4 KDa, respectively. Each EPP and IP were tested in vitro for nematicide and nematostatic activity against <i>M. incognita</i> and <i>M. javanica,</i> through motility and mortality evaluation. Regarding resistance induction assays, root samples were also collected for the analysis of defense enzymes peroxidase (POX), polyphenoloxidase (POL) and phenylalanine ammonia-lyase (FAL). It is possible to conclude that <i>P. sanguineus</i> basidiocarps contain proteins with no nematicide/nematostatic effects but are capable of reducing <i>M. javanica</i> infection in Micro-Tom tomato plants, which can occur by resistance induction involving peroxidase activity.</p>

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Proteins of Pycnoporus sanguineus have nematicide activity and induce resistance to Meloidogyne spp. in Micro-Tom tomato plants

  • 期刊论文

  • 发表日期：

    2025年5月20日

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  • Olivia Diulen Costa Brito,
  • Bruna Caroline Schons,
  • Roberto Cecatto Junior,
  • Diego Borges Dalevedove,
  • Juliana Yuriko Habitzreuter Fujimoto,
  • José Renato Stangarlin

摘要

The aim of this research was to obtain protein-fractions from the aqueous extract of the fungus P. sanguineus through gel filtration chromatography (GFC) to induce resistance to Meloidogyne spp. in Micro-Tom tomato plants. Precipitation of protein from the aqueous extract (AE) of basidiocarps was performed based on increasing ethanol gradients 20%, 40%, 60%, 80% and 95%, which were individually centrifuged. Each ethanol-protein precipitate (EPP) was fractionated by using GFC, and isolated proteins (IP) were identified based on molecular mass. Six IP (named as I, II, III, IV, V and VI) were obtained through GFC, whose molecular weights were 4.81, 47.98, 0.17, 25.78, 4.25 and 4 KDa, respectively. Each EPP and IP were tested in vitro for nematicide and nematostatic activity against M. incognita and M. javanica, through motility and mortality evaluation. Regarding resistance induction assays, root samples were also collected for the analysis of defense enzymes peroxidase (POX), polyphenoloxidase (POL) and phenylalanine ammonia-lyase (FAL). It is possible to conclude that P. sanguineus basidiocarps contain proteins with no nematicide/nematostatic effects but are capable of reducing M. javanica infection in Micro-Tom tomato plants, which can occur by resistance induction involving peroxidase activity.