<p>Tomato cultivation in hot and humid regions is affected by bacterial infections, with bacterial spot posing a major threat by cultivation unviable, as it can easily spread and is difficult to control. This study aimed to evaluate the inactivated bacterial suspension (IBS) as an attenuator for controlling bacterial spot (<i>Xanthomonas euvesicatoria</i> pv. <i>perforans)</i> in dwarf tomato lines. The experiment was conducted in a factorial scheme with seven genotypes, including four dwarf tomato lines, the resistant wild accession <i>Solanum pennellii</i>, the susceptible commercial cultivar Santa Clara and the parent UFU MC TOM1, alongside four products [IBS, copper, acibenzolar-S-methyl (ASM), and water], in randomized blocks with four replicates. The isolate UFU B8 of <i>X</i>. <i>euvesicatoria</i> pv. <i>perforans</i> was used, with IBS obtained through autoclave sterilization. The products were applied 2 and 4 days before inoculation with a <i>X</i>. <i>euvesicatoria</i> pv. <i>perforans</i> bacterial suspension (10⁷ CFU.mL^-1). Disease severity was evaluated at 3, 6, 9 and 12 days after inoculation, and the area under the disease progress curve (AUDPC) was calculated. Chromatographic analysis and metabolomic profile were also evaluated. Variance analysis and mean comparisons were conducted using the Scott-Knott test (<i>p</i> &lt; 0.05). Multivariate analysis was performed and the dendrogram, selection index, and relative superiority were obtained, forming four distinct groups. Group IV, consisting of lines 1, 2, and the parent UFU MC TOM1, was selected using the genotype-ideotype index and exhibited superior resistance, when compared to <i>S</i>. <i>pennellii</i>. ASM, IBS, and copper treatments effectively reduced bacterial spot severity on tomato plants. The most remarkable metabolites were myo-inositol, oxalic acid and glycoside. The highest intensity of these metabolites was found for treatments with UFU MC TOM1.</p>