Scholarlink

<p>Infection of coffee leaves by the biotrophic fungus <i>Hemileia vastatrix</i> results in intense plant defoliation and lower fruit berries production. Resistant cultivars and fungicide spray (contact and/or systemic) help to reduce the impact of coffee leaf rust (CLR) on coffee production. New alternatives for CLR management are needed to reduce fungicides spray and the emergence of populations from <i>H. vastatrix</i> resistant to them. In this study, it was hypothesized that <i>β</i>-aminobutyric acid (BABA) could boost the defense of coffee leaves against infection by <i>H. vastatrix</i>. In the <i>in vitro</i> assay, urediniospores germination was significantly reduced from 58 to 94% as the concentrations of BABA increased from 100 to 500&#xa0;mM. Fungal sporulation and uredinia were much less intense on the leaves of BABA-sprayed plants compared to the ones obtained from water-sprayed plants at 22 and 30&#xa0;days after inoculation. The area under coffee leaf rust progress curve and intensity of fungal sporulation were significantly lower by 76 and 88%, respectively, for BABA-sprayed plants compared to water-sprayed plants. The BABA-sprayed plants infected by <i>H. vastatrix</i> showed better photosynthetic performance, greater concentrations of chlorophyll <i>a</i> + <i>b</i> and carotenoids, higher activity of defense-related enzymes (chitinase and phenylalanine ammonia-lyase), and a more robust antioxidative metabolism (higher activities of ascorbate peroxidase, catalase, glutathione reductase, and superoxide dismutase) compared to water-sprayed and infected ones. Higher concentration of superoxide anion radical on BABA-sprayed and infected leaves intensified the defense against infection by <i>H. vastatrix</i> besides having a possible fungistatic effect against the fungus. In conclusion, BABA besides affecting the germination of urediniospores from <i>H. vastatrix</i> helped them to cope more efficiently against infection by <i>H. vastatrix</i> and can become an alternative for CLR control.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

β-aminobutyric acid-induced resistance in coffee plants enhances defense against infection by Hemileia vastatrix

  • 期刊论文

  • 发表日期：

    2025年2月10日

查看全文
  • Verônica Vieira Brás,
  • Leandro Castro Silva,
  • Bruno Nascimento Silva,
  • Leonardo Packer Quadros,
  • Henara Valéria Miranda Castro,
  • Fabrício Ávila Rodrigues

摘要

Infection of coffee leaves by the biotrophic fungus Hemileia vastatrix results in intense plant defoliation and lower fruit berries production. Resistant cultivars and fungicide spray (contact and/or systemic) help to reduce the impact of coffee leaf rust (CLR) on coffee production. New alternatives for CLR management are needed to reduce fungicides spray and the emergence of populations from H. vastatrix resistant to them. In this study, it was hypothesized that β-aminobutyric acid (BABA) could boost the defense of coffee leaves against infection by H. vastatrix. In the in vitro assay, urediniospores germination was significantly reduced from 58 to 94% as the concentrations of BABA increased from 100 to 500 mM. Fungal sporulation and uredinia were much less intense on the leaves of BABA-sprayed plants compared to the ones obtained from water-sprayed plants at 22 and 30 days after inoculation. The area under coffee leaf rust progress curve and intensity of fungal sporulation were significantly lower by 76 and 88%, respectively, for BABA-sprayed plants compared to water-sprayed plants. The BABA-sprayed plants infected by H. vastatrix showed better photosynthetic performance, greater concentrations of chlorophyll a + b and carotenoids, higher activity of defense-related enzymes (chitinase and phenylalanine ammonia-lyase), and a more robust antioxidative metabolism (higher activities of ascorbate peroxidase, catalase, glutathione reductase, and superoxide dismutase) compared to water-sprayed and infected ones. Higher concentration of superoxide anion radical on BABA-sprayed and infected leaves intensified the defense against infection by H. vastatrix besides having a possible fungistatic effect against the fungus. In conclusion, BABA besides affecting the germination of urediniospores from H. vastatrix helped them to cope more efficiently against infection by H. vastatrix and can become an alternative for CLR control.