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Purpose <p>To explore the effects of bone marrow mesenchymal stem cell-derived exosomes on the proliferation and migration of chondrocytes cultured in vitro.</p> Methods <p>Bone marrow mesenchymal stem cells (BMSCs) and BMSC-derived exosomes (BMSC Exos) were obtained, and their surface markers were identified based on microstructure and protein marker phenotype. A CCK-8 assay was performed to detect the effects of different concentrations. A scratch test was used to analyze the ability of BMSC Exos to regulate chondrocyte migration.</p> Results <p>The obtained BMSC Exos exhibited an approximately oval shape. The BMSC-derived exosomes obtained by ultracentrifugation were analyzed by NTA, and the particle sizes were mostly between 80&#xa0;nm and 200&#xa0;nm, with a main peak at approximately 112&#xa0;nm. Western blot analysis indicated that these exosomes expressed exosomal surface markers, including CD9, CD63 and CD81. The results of the scratch experiments revealed that the exosomes could significantly promote the healing of scratched chondrocytes, and the healing speed was significantly accelerated with increasing exosome concentration and prolonged treatment time.</p> Conclusion <p>BMSC-derived exosomes can promote the proliferation and migration of chondrocytes cultured in vitro, making it possible to replace BMSCs as a novel noncellular therapy method.</p>

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Effects of BMSC-Exosomes on the Proliferation and Migration of Chondrocytes

  • 期刊论文

  • 发表日期：

    2025年1月15日

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  • Kai Sun,
  • Meng Fan

摘要

Purpose

To explore the effects of bone marrow mesenchymal stem cell-derived exosomes on the proliferation and migration of chondrocytes cultured in vitro.

Methods

Bone marrow mesenchymal stem cells (BMSCs) and BMSC-derived exosomes (BMSC Exos) were obtained, and their surface markers were identified based on microstructure and protein marker phenotype. A CCK-8 assay was performed to detect the effects of different concentrations. A scratch test was used to analyze the ability of BMSC Exos to regulate chondrocyte migration.

Results

The obtained BMSC Exos exhibited an approximately oval shape. The BMSC-derived exosomes obtained by ultracentrifugation were analyzed by NTA, and the particle sizes were mostly between 80 nm and 200 nm, with a main peak at approximately 112 nm. Western blot analysis indicated that these exosomes expressed exosomal surface markers, including CD9, CD63 and CD81. The results of the scratch experiments revealed that the exosomes could significantly promote the healing of scratched chondrocytes, and the healing speed was significantly accelerated with increasing exosome concentration and prolonged treatment time.

Conclusion

BMSC-derived exosomes can promote the proliferation and migration of chondrocytes cultured in vitro, making it possible to replace BMSCs as a novel noncellular therapy method.