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<p>The real-time, <i>in situ</i> imaging of hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>), a key reactive oxygen species implicated in various diseases, remains challenging. This is primarily due to the limitations of existing probes, such as short emission wavelengths and the reliance on external excitation. To address these issues, we developed an H<sub>2</sub>O<sub>2</sub>-triggered near-infrared (NIR) chemiluminescence (CL) nanoprobe with aggregation-induced emission (AIE) characteristics for <i>in vivo</i> inflammation imaging and tumor theranostics. This nanoprobe, denoted as CPPO@TN NPs, was constructed by co-encapsulating a tailored AIE photosensitizer (TN) with strong NIR emission and high singlet oxygen (<sup>1</sup>O<sub>2</sub>) generation, a H<sub>2</sub>O<sub>2</sub>-responsive chemiluminescent substrate (CPPO), and soybean oil (as a retarder) within F-127 micelles. Upon encountering H<sub>2</sub>O<sub>2</sub>, the nanoprobe undergoes a persistent chemically initiated electron exchange luminescence (CIEEL) process that activates AIEgens, resulting in intense NIR chemiluminescence and sustained <sup>1</sup>O<sub>2</sub> production without the need for external irradiation. Leveraging this mechanism, CPPO@TN NPs achieved highly sensitive and specific imaging of drug-induced liver injury and peritonitis in murine models, with exceptional tissue penetration and signal-to-noise ratio. Furthermore, the nanoprobe facilitated effective self-luminescent imaging and photodynamic therapy of tumors, significantly inhibiting tumor growth in a 4T1 tumor-bearing mouse model. This platform provides an external light excitation-free theranostic strategy for H<sub>2</sub>O<sub>2</sub>-associated diseases.</p>

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A H2O2-triggered NIR chemiluminescence nanoprobe with aggregation-induced emission properties for in vivo inflammation imaging and tumor theranostics

  • 期刊论文

  • 发表日期：

    2026年7月7日

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  • Junhao Huang,
  • Lin Yang,
  • Yufeng Xiao,
  • Yuxi Li,
  • Jiachang Huang,
  • Ben Zhong Tang,
  • Benzhao He

摘要

The real-time, in situ imaging of hydrogen peroxide (H2O2), a key reactive oxygen species implicated in various diseases, remains challenging. This is primarily due to the limitations of existing probes, such as short emission wavelengths and the reliance on external excitation. To address these issues, we developed an H2O2-triggered near-infrared (NIR) chemiluminescence (CL) nanoprobe with aggregation-induced emission (AIE) characteristics for in vivo inflammation imaging and tumor theranostics. This nanoprobe, denoted as CPPO@TN NPs, was constructed by co-encapsulating a tailored AIE photosensitizer (TN) with strong NIR emission and high singlet oxygen (1O2) generation, a H2O2-responsive chemiluminescent substrate (CPPO), and soybean oil (as a retarder) within F-127 micelles. Upon encountering H2O2, the nanoprobe undergoes a persistent chemically initiated electron exchange luminescence (CIEEL) process that activates AIEgens, resulting in intense NIR chemiluminescence and sustained 1O2 production without the need for external irradiation. Leveraging this mechanism, CPPO@TN NPs achieved highly sensitive and specific imaging of drug-induced liver injury and peritonitis in murine models, with exceptional tissue penetration and signal-to-noise ratio. Furthermore, the nanoprobe facilitated effective self-luminescent imaging and photodynamic therapy of tumors, significantly inhibiting tumor growth in a 4T1 tumor-bearing mouse model. This platform provides an external light excitation-free theranostic strategy for H2O2-associated diseases.